HCAR2 蛋白, Human (Cell-Free, His)

HCAR2 protein, a high-affinity receptor for nicotinic acid and (D)-beta-hydroxybutyrate, induces increased adiponectin secretion and reduced lipolysis through G(i)-protein-mediated adenylyl cyclase inhibition. This requires nicotinic acid doses exceeding typical dietary levels. HCAR2 also mediates nicotinic acid-induced apoptosis in neutrophils. Upon nicotinic acid activation, reduced cAMP levels potentially influence cAMP-dependent protein kinase A activity and target protein phosphorylation, leading to neutrophil apoptosis. Relative potency for displacing nicotinic acid binding: 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. HCAR2 Protein, Human (Cell-Free, His) is the recombinant human-derived HCAR2 protein, expressed by E. coli Cell-free, with C-6*His labeled tag. The total length of HCAR2 Protein, Human (Cell-Free, His) is 363 a.a., with molecular weight of 42.7 kDa.

HCAR2 蛋白作为烟酸（烟酸）和 (D)-β-羟基丁酸的高亲和力受体，通过 G(i) 蛋白介导的腺苷酸环化酶抑制来协调增加脂联素分泌并减少脂肪分解。这种药理作用需要烟酸剂量超过通常从常规饮食中获得的剂量。此外，HCAR2 介导烟酸诱导的成熟中性粒细胞凋亡。烟酸激活受体后，cAMP 水平随之降低，可能影响 cAMP 依赖性蛋白激酶 A 的活性和靶蛋白的磷酸化，最终导致中性粒细胞凋亡。取代烟酸结合的相对效力遵循以下顺序：5-甲基吡唑-3-羧酸=吡啶-3-乙酸>阿西夫兰>5-甲基烟酸=阿西莫司>>烟尿酸=烟酰胺。

Human

E. coli Cell-free

C-6*His

Q8TDS4 (M1-P363)

338442

MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSGEPWSPSYLGPTSP

42.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.