2. 重组蛋白
  3. Enzymes & Regulators Viral Proteins
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  5. Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain RW (Cell-Free, His)

Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain RW (Cell-Free, His)

目录号: HY-P702319
产品使用指南

血凝素神经氨酸酶 (HN) 蛋白在病毒感染中至关重要，它将病毒附着在含唾液酸的细胞受体上，引发感染。它的结合会引起构象变化，使 F 蛋白能够触发病毒粒子-细胞膜融合，从而促进病毒进入。HN 蛋白 的神经氨酸酶活性通过将成熟病毒颗粒与含有神经氨酸的糖蛋白分离来确保病毒的有效传播。Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain RW (Cell-Free, His) 是重组的 血凝素神经氨酸酶/HN 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain RW (Cell-Free, His) 全长 582 个氨基酸，分子量为 65.6 kDa。

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描述

Hemagglutinin neuraminidase (HN) Protein is pivotal in viral infection, attaching the virus to sialic acid-containing cell receptors, initiating infection. Its binding induces a conformational change enabling the F protein to trigger virion-cell membrane fusion, facilitating virus entry. HN Protein's neuraminidase activity ensures efficient virus spread by dissociating mature virions from glycoproteins containing neuraminic acid. Hemagglutinin neuraminidase/HN Protein, Mumps virus strain RW (Cell-Free, His) is the recombinant Virus-derived Hemagglutinin neuraminidase/HN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Hemagglutinin neuraminidase/HN Protein, Mumps virus strain RW (Cell-Free, His) is 582 a.a., with molecular weight of 65.6 kDa.
研究背景

血凝素神经氨酸酶 (HN) 蛋白在病毒感染中发挥着至关重要的作用，它可以将病毒附着在含有唾液酸的细胞受体上，从而启动感染过程。当HN蛋白与受体结合时，它会诱导构象变化，使F蛋白能够触发病毒与细胞膜之间的融合，从而促进病毒进入宿主细胞。此外，HN 蛋白具有神经氨酸酶活性，可通过将成熟病毒粒子与含有神经氨酸的糖蛋白解离来确保病毒的有效传播。
种属

Virus
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

P10866 (M1-T582)
基因 ID

/
蛋白结构
N-term
10*His
HN (M1-T582)
Accession # P10866
C-term
同用名
Hemagglutinin-neuraminidase
氨基酸序列

MEPSKLFTISDNATFAPGPVNNAADKKTFRTCFRILVLSVQAVTLILVIVTLGELVRMINDQGLSNQLSSITDKIRESATMIASAVGVMNQVIHGVTVSLPLQIEGNQNQLLSTLATICTSKKQISNCSTNIPLVNDLRFINGINKFIIEDYANHDFSIGHPLNMPSFIPTATSPNGCTRIPSFSLGKTHWCYTHNVINANCKDHTSSNQYVSMGILVQTASGYPMFKTLKIQYLSDGLNRKSCSIATVPDGCAMYCYVSTQLETDDYAGSSPPTQKLTLLFYNDTVTERTISPSGLEGNWATLVPGVGSGIYFENKLIFPAYGGVLPNSTLGVKLAREFFRPVNPYNPCSGPQQDLDQRALRSYFPSYLSNRRVQSAFLVCAWNQILVTNCELVVPSNNQTLMGAEGRVLLINNRLLYYQRSTSWWPYELLYEISFTFTNSGQSSVNMSWIPIYSFTRPGSGKCSGENVCPIACVSGVYLDPWPLTPYSHQSGINRNFYFTGALLNSSTTRVNPTLYVSALNNLKVLAPYGTQGLSASYTTTTCFQDTGDASVYCVYIMELASNIVGEFQILPVLTRLTIT

分子量

65.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Hemagglutinin neuraminidase/HN Protein, Mumps virus strain RW (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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