1. 重组蛋白
  2. Enzymes & Regulators Viral Proteins
  3. Hydrolases (EC 3)
  4. Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain SBL-1 (Cell-Free, His)

Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain SBL-1 (Cell-Free, His)

目录号: HY-P702318
产品使用指南

血凝素神经氨酸酶(HN)蛋白是病毒感染的关键,它将病毒附着在含唾液酸的细胞受体上并引发感染。它的结合促使结构发生变化,使 F 蛋白能够启动病毒粒子-细胞膜融合,从而促进病毒进入。Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain SBL-1 (Cell-Free, His) 是重组的 血凝素神经氨酸酶/HN 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。Hemagglutinin neuraminidase/HN 蛋白, Mumps virus strain SBL-1 (Cell-Free, His) 全长 582 个氨基酸,分子量为 65.4 kDa。

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描述

The hemagglutinin-neuraminidase (HN) protein is key to viral infection, attaching the virus to sialic acid-containing cell receptors and initiating infection. Its binding prompts structural changes that enable the F protein to initiate virion-cell membrane fusion, thereby facilitating viral entry. Hemagglutinin neuraminidase/HN Protein, Mumps virus strain SBL-1 (Cell-Free, His) is the recombinant Virus-derived Hemagglutinin neuraminidase/HN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Hemagglutinin neuraminidase/HN Protein, Mumps virus strain SBL-1 (Cell-Free, His) is 582 a.a., with molecular weight of 65.4 kDa.

研究背景

血凝素神经氨酸酶 (HN) 蛋白是病毒感染的关键参与者。它促进病毒附着到含有唾液酸的细胞受体上,从而启动感染过程。当HN蛋白与受体结合时,会引发结构变化,使F蛋白能够启动病毒与细胞膜之间的融合,从而促进病毒进入宿主细胞。此外,HN蛋白具有神经氨酸酶活性,这在病毒的有效传播中起着至关重要的作用。这种活性有助于将成熟病毒颗粒与含有神经氨酸的糖蛋白分离,从而使病毒能够有效繁殖。

种属

Virus

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P19762 (M1-T582)

基因 ID

/

蛋白结构
N-term
10*His
HN (M1-T582)
Accession # P19762
C-term
同用名
Hemagglutinin-neuraminidase
氨基酸序列

MEPSKLFIMSDNATVAPGPVVNAAGKKTFRTCFRILVLSVQAVTLILVIVTLGELIRMINDQGLSNQLSSITDKIRESAAVIASAVGVMNQVIHGVTVSLPLQIEGNQNQLLSTLATICTNRNQVSNCSTNIPLVNDLRFINGINKFIIEDYATHDFSIGNPLNMPSFIPTATSPNGCTRIPSFSLGKTHWCYTHNVINANCKDHTSSNQYVSMGILVQTASGYPMFKTLKIQYLSDGLNRKSCSIATVPDGCAMYCYVSTQLEANDYAGSSPPTQKLTLLFYNDTITERTISPSGLEGNWATLVPGVGSGIYFENKLIFPAYGGVLPNSTLGVKSAREFFRPVNPYNPCSGPPQELDQRALRSYFPRYFSSRRVQSAFLVCAWNQILVTNCELVVPSNNQTLMGAEGRVLLINNRLLYYQRSTSWWPYELLYEISFTFTNSGQSSVNMSWIPIYSFTPPGSGNCSGKNVCPTVCVSGVYLDPWPLTPYSHQSGINRNFYFTGALLNSSTTRVNPTLYVSALNNLKVLAPYGTQGLFASYTTTTCFQDTGDASVYCVYIMELASNIVGEFQILPVLARLTIT

分子量

65.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Hemagglutinin neuraminidase/HN Protein, Mumps virus strain SBL-1 (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Hemagglutinin neuraminidase/HN Protein, Mumps virus strain SBL-1 (Cell-Free, His)
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