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  3. Enzymes & Regulators Viral Proteins
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  5. Hemagglutinin neuraminidase/HN 蛋白, Newcastle disease virus (Cell-Free, His)

Hemagglutinin neuraminidase/HN 蛋白, Newcastle disease virus (Cell-Free, His)

目录号: HY-P702320
产品使用指南

血凝素神经氨酸酶 (HN) 蛋白通过将病毒附着到细胞受体并诱导与宿主膜融合来促进病毒进入。它与融合 (F) 蛋白一起作用，通过将成熟病毒颗粒与糖蛋白分离来实现病毒的传播。HN 在感染和传播中的关键作用使其对于病毒的成功至关重要。Hemagglutinin neuraminidase/HN 蛋白, Newcastle disease virus (Cell-Free, His) 是重组的 血凝素神经氨酸酶/HN 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。Hemagglutinin neuraminidase/HN 蛋白, Newcastle disease virus (Cell-Free, His) 全长 571 个氨基酸，分子量为 68.7 kDa。

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描述

The Hemagglutinin Neuraminidase (HN) protein facilitates viral entry by attaching the virus to cell receptors and inducing fusion with host membranes. It works with the fusion (F) protein and enables the spread of the virus by dissociating mature virions from glycoproteins. HN's crucial role in infection and propagation makes it essential for viral success. Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His) is the recombinant Virus-derived Hemagglutinin neuraminidase/HN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His) is 571 a.a., with molecular weight of 68.7 kDa.
研究背景

血凝素神经氨酸酶 (HN) 蛋白在病毒进入过程中发挥着至关重要的作用，它与融合 (F) 蛋白协同作用，介导病毒进入宿主细胞。HN 将病毒附着在含唾液酸的细胞受体上，启动感染过程。与受体结合后，HN 蛋白会诱导构象变化，使 F 蛋白能够触发病毒粒子和细胞膜的融合。此外，HN 的神经氨酸酶活性有助于促进病毒的有效传播。该活性负责将成熟病毒颗粒与含神经氨酸的糖蛋白分离，从而有助于病毒繁殖的总体成功。
种属

Virus
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

P35741 (M1-V571)
基因 ID

/
蛋白结构
N-term
10*His
HN (M1-V571)
Accession # P35741
C-term
同用名
Hemagglutinin-neuraminidase
氨基酸序列

MDRAVSRVALENEEREAKNTWRFVFRIAILLLIVITLAISAAALVYSMEASTPGDLVGIPTVISRAEEKITSALSSNQDVVDRIYKQVALESPLALLNTESVIMNAITSLSYQINGAANNSGCGAPVHDPDYIGGIGKELIVDDASDVTSFYPSAFQEHLNFIPAPTTGSGCTRIPSFDISATHYCYTHNVILSGCRDHSHSHQYLALGVLRTSATGRVFFSTLRSINLDDNQNRKSCSVSATPLGCDMLCSKITETEEEDYSSVTPTSMVHGRLGFDGQYHEKDLDVITLFKDWVANYPGVGGGSFIDNRVWFPVYGGLKPNSPSDTVQEGRYVIYKRYNDTCPDEQDYQIRMAKSSYKPGRFGGKRVQQAILSIKVSTSLGEDPVLTIPPNTVTLMGAEGRVLTVGTSHFLYQRGSSYFSPALLYPMTVNNKTATLHSPYTFNAFTRPGSVPCQASARCPNSCVTGVYTDPYPLIFHRNHTLRGVFGTMLDDGQARLNPVSAVFDNISRSRITRVSSSRTKAAYTTSTCFKVVKTNKTYVLSIAEISNTLFGEFRIVPLLVEILKNDGV

分子量

68.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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