HSP70/DnaK 蛋白, E. coli (P.pastoris, His)

HSP70/DnaK protein, a molecular chaperone, responds primarily to stress, notably heat shock. It plays a crucial role in aiding the proper folding of proteins during cellular stress, preventing misfolding or aggregation. The chaperone activity is vital for cellular homeostasis and survival under adverse conditions, where maintaining correct protein conformation becomes challenging. HSP70/DnaK's ability to recognize and assist in refolding misfolded or denatured proteins is essential for cellular resilience, preventing proteotoxicity induced by stressors, especially elevated temperatures. HSP70/DnaK Protein, E. coli (P.pastoris, His) is the recombinant E. coli-derived HSP70/DnaK protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of HSP70/DnaK Protein, E. coli (P.pastoris, His) is 638 a.a., with molecular weight of ~71.1 kDa.

HSP70/DnaK 蛋白作为分子伴侣，主要响应热休克等应激条件。在细胞应激时，这种蛋白质在协助其他蛋白质正确折叠、防止错误折叠或聚集方面发挥着至关重要的作用。HSP70/DnaK 的伴侣活性对于细胞稳态和在不利环境条件下的生存尤其重要，在这种条件下维持蛋白质的正确构象变得具有挑战性。该蛋白质识别并协助错误折叠或变性蛋白质重折叠的能力对于细胞弹性和预防各种应激源（特别是高温）引起的蛋白质毒性至关重要。

E.coli

P. pastoris

N-6*His

Q1RGI8 (M1-K638)

58463508  [NCBI]

MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK

Approximately 71.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.