1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-36
  5. IL-36 alpha
  6. IL-36 alpha/IL-1F6 蛋白, Human (153a.a)

IL-36 alpha/IL-1F6 蛋白, Human (153a.a)

目录号: HY-P70702
COA 产品使用指南

IL-36 alpha (IL-1F6) 是 IL-36 家族的其中一个亚型,属于 IL-1 超家族。IL-36 alpha 介导炎症反应。IL-36 alpha 与 IL-36R 结合并激活 NF-κB 和 MAPK 信号通路,但这种激活需要中性粒细胞来源的蛋白酶对 IL-36 alpha 的 N 端进行切割。IL-36 alpha 还可以结合 IL-1Rrp2 并募集 IL-1RAcP。IL-36 alpha 通过 IL-36R/IL-1RAcP 激活 MAPK、Erk1/2 和 JNK 信号通路。IL-36 alpha/IL-1F6 蛋白, Human (153a.a) 是一种由 E. coli 产生的重组人 IL-36 alpha (K6-F158),不带任何标签。

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50 μg ¥5100 In-stock
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描述

IL-36 alpha (IL-1F6), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 alpha mediates inflammatory response. IL-36 alpha binds to IL-36R and activates NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases[1]. IL-36 alpha also binds to IL-1Rrp2 and recruit IL-1RAcP. IL-36 alpha activats the MAPK, Erk1/2 and JNK through IL-36R/IL-1RAcP[2]. IL-36 alpha/IL-1F6 Protein, Human (153a.a) is a recombinant human IL-36 alpha (K6-F158) without any tag, which is produced in E. coli.

研究背景

IL-36 alpha, a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 alpha is expressed in monocytes, T/B-lymphocytes, spleen, bone-marrow tonsils, lymph nodes and skin[2]. IL-36 alpha is up-regulated in injured kidney. IL-36 alpha is associated with the development of renal pathologies, as well as hepatocellular carcinoma, and some inflammatory/immune diseases including colitis and psoriasis[3].
The sequence of amino acids in IL-36 alpha differs in different species. Human IL-36 alpha shares <55% aa sequence identity with mouse.
IL-36 alpha binds to IL-36R and activates NF-κB and MAPK signaling pathways, thereby mediating inflammatory response. But the activation requires N-terminal cleavage by neutrophil granule-derived proteases, such as cathepsin G, elastase and proteinase-3[1]. IL-36 alpha can also bind IL-1Rrp2 and recruit IL-1RAcP. IL-36 alpha activats the MAPK, Erk1/2 and JNK through IL-36R/IL-1RAcP[2].
IL-36 alpha is a pro-inflammatory factor. IL-36 alpha mediates inflammatory response through the activation of NF-κB and MAPK signaling pathway[1].

体外研究
(In Vitro)

IL-36 alpha (human) (10 ng/mL, 48 h) stimulates the production of TNF-α and IL-1β in HCECs[4].
IL-36 alpha (human) (500 ng/mL, 48 h) induces maturation of human MDDCs[5].
IL-36 alpha (human) inhibits proliferation, migration, and invasion of both OV2008 and SKOV-3 cells[6].

生物活性

The cell proliferation assay using A-431 Human epithelial carcinoma cells has an ED50 value of 12 ng/mL.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9UHA7 (K6-F158)

基因 ID

27179  [NCBI]

中文名
重组人 IL-36 alpha/IL-1F6 (153a.a)
同用名
IL-36 alpha; IL-36α; Interleukin-36 Alpha; FIL1 Epsilon; Interleukin-1 Epsilon; IL-1 Epsilon; Interleukin-1 Family Member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6
氨基酸序列

KIDTPQQGSIQDINHRVWVLQDQTLIAVPRKDRMSPVTIALISCRHVETLEKDRGNPIYLGLNGLNLCLMCAKVGDQPTLQLKEKDIMDLYNQPEPVKSFLFYHSQSGRNSTFESVAFPGWFIAVSSEGGCPLILTQELGKANTTDFGLTMLF

分子量

Approximately 17.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

IL-36 alpha/IL-1F6 Protein, Human (153a.a) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-36 alpha/IL-1F6 Protein, Human (153a.a)
目录号:
HY-P70702
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