JAM-B/CD322 蛋白, Rat (HEK293, His)

Junctional adhesion molecule 2 (Jam2, Jam-B, CD322) is a member of the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. Jam2 is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. Jam2 has integrin alpha-4/beta-1 binding activity and an inhibitory somatodendritic cue. JAM-B/CD322 Protein, Rat (HEK293, His) is the recombinant rat-derived JAM-B/CD322 protein, expressed by HEK293 , with C-His labeled tag. The total length of JAM-B/CD322 Protein, Rat (HEK293, His) is 208 a.a., with molecular weight (glycosylation form) of ~30-35 kDa.

连接粘附分子 2 (Jam2, JAM-b, CD322) 是免疫球蛋白超家族的成员，也是连接粘附分子 (JAM) 家族的成员。Jam2 是一种 I 型膜蛋白，定位于上皮细胞和内皮细胞的紧密连接处。Jam2 作为一种粘附配体与多种免疫细胞类型相互作用，并可能在淋巴细胞归巢到次级淋巴器官中发挥作用。Jam2 还具有整合素 α-4/β-1 结合活性，并在许多细胞过程中发挥重要作用，如紧密连接组装、精子发生、细胞旁通透性调节、白细胞迁移、血管生成、肿瘤转移和细胞增殖。此外，Jam2 作为抑制性体树突信号，阻止神经元非轴突部分的髓鞘形成，有助于肌肉形成过程中的肌细胞融合^[1][2][3]。

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells. When 8 × 10⁴ cells/well are added to CD322-coated plates (5 μg/mL and 100 μL/well), approximately 43.87% will adhere specifically after 60 minutes at 37°C.

Rat

HEK293

C-His

Q3MHC0 (F29-N236)

619374  [NCBI]

FSASKDHRQEVSVIEYQEAILACKTPKKTTSSRLEWKKLGQGVSLVYYQQALQGDFKDRAEMIDFNIRIKNVTRHDAGEYRCEVSAPTEQGQNLQEDTVMLEVLVAPAVPSCEVPTSVMSGSVVELRCQEKEGNPAPEYIWFKDGTSLLGNPKGGAHRNSSYTMNTKSGTLQFNMISKMDSGEYYCEARNSVGHRRCPGKRMQVDVLN

Approximately 30-35 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE. 320008

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.