2. 重组蛋白
  3. Others
  4. KCSA 蛋白, Streptomyces coelicolor (Cell-Free, His)

KCSA 蛋白, Streptomyces coelicolor (Cell-Free, His)

目录号: HY-P702351
产品使用指南

KCSA 蛋白是一种 pH 门控钾离子通道，独特地响应细胞质 pH 变化（特别是从 7 到 4）而打开。这种同源四聚体分子守门者促进钾离子的 pH 依赖性通过。KCSA 蛋白, Streptomyces coelicolor (Cell-Free, His) 是重组的 KCSA 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。KCSA 蛋白, Streptomyces coelicolor (Cell-Free, His) 全长 160 个氨基酸，分子量为 23.7 kDa。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

KCSA Protein, a pH-gated potassium ion channel, uniquely opens in response to cytosolic pH changes, particularly from 7 to 4. This homotetrameric molecular gatekeeper facilitates pH-dependent passage of potassium ions. KCSA Protein, Streptomyces coelicolor (Cell-Free, His) is the recombinant KCSA protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of KCSA Protein, Streptomyces coelicolor (Cell-Free, His) is 160 a.a., with molecular weight of 23.7 kDa.
研究背景

KCSA 蛋白充当 pH 门控钾离子通道，表现出响应胞质 pH 变化而打开的独特能力，当 pH 从 7 变为 4 时，通道打开。这种同源四聚体蛋白充当分子看门人，允许钾离子以 pH 依赖性方式通过。
种属

Others
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

P0A333 (M1-R160)
基因 ID

/
蛋白结构
N-term
10*His
KCSA (M1-R160)
Accession # P0A333
C-term
同用名
pH-gated potassium channel KcsA
氨基酸序列

MPPMLSGLLARLVKLLLGRHGSALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRLVAVVVMVAGITSFGLVTAALATWFVGREQERRGHFVRHSEKAAEEAYTRTTRALHERFDRLERMLDDNRR

分子量

23.7 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

KCSA Protein, Streptomyces coelicolor (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
KCSA Protein, Streptomyces coelicolor (Cell-Free, His)
目录号：
HY-P702351
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z