1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. LCLAT1 蛋白, Human (Cell-Free, His)

LCLAT1 蛋白, Human (Cell-Free, His)

目录号: HY-P702357
产品使用指南

LCLAT1 蛋白是一种酰基辅酶 A:溶心磷脂酰基转移酶 (ALCAT),在重塑过程中至关重要地催化溶心磷脂再酰化为心磷脂 (CL)。它显示出底物灵活性,优选亚油酰辅酶A和油酰辅酶A作为单心磷脂和双心磷脂的酰基供体。LCLAT1 蛋白, Human (Cell-Free, His) 是重组的 LCLAT1 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。LCLAT1 蛋白, Human (Cell-Free, His) 全长 414 个氨基酸,分子量为 55.0 kDa。

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描述

The LCLAT1 protein is an acyl-CoA:lysocardiolipin acyltransferase (ALCAT) that crucially catalyzes the reacylation of lyscardiolipin to cardiolipin (CL) during remodeling. It shows substrate flexibility, preferring linoleyl-CoA and oleoyl-CoA as acyl donors for mono- and di-cardiolipins. LCLAT1 Protein, Human (Cell-Free, His) is the recombinant human-derived LCLAT1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of LCLAT1 Protein, Human (Cell-Free, His) is 414 a.a., with molecular weight of 55.0 kDa.

研究背景

LCLAT1 蛋白作为酰基辅酶 A:溶血心磷脂酰基转移酶 (ALCAT),在催化溶血心磷脂再酰化为心磷脂 (CL) 中发挥着至关重要的作用,这是 CL 重塑的关键步骤。该酶具有底物灵活性,可识别单溶心磷脂和二溶心磷脂,并优先选择亚油酰辅酶 A 和油酰辅酶 A 作为酰基供体。此外,LCLAT1 具有 1-酰基-sn-甘油-3-磷酸酰基转移酶活性 (AGPAT),可将 1-酰基-sn-甘油-3-磷酸(溶血磷脂酸或 LPA)转化为 1,2-二酰基-sn-甘油- 3-磷酸酯(磷脂酸或 PA),通过在甘油主链的 sn-2 位置掺入酰基部分来实现。此外,它还具有溶血磷脂酰肌醇酰基转移酶(LPIAT)和溶血磷脂酰甘油酰基转移酶(LPGAT)活性。除了其酶功能外,LCLAT1 对于造血和内皮谱系的建立至关重要,凸显了其在发育过程中的关键作用。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q6UWP7 (M1-E414)

基因 ID

253558

蛋白结构
N-term
10*His
LCLAT1 (M1-E414)
Accession # Q6UWP7
C-term
同用名
Lysocardiolipin acyltransferase 1; 1-acylglycerol-3-phosphate O-acyltransferase 8; 1-AGP acyltransferase 8; 1-AGPAT 8; 2.3.1.51; Acyl-CoA:lysocardiolipin acyltransferase 1
氨基酸序列

MHSRGREIVVLLNPWSINEAVSSYCTYFIKQDSKSFGIMVSWKGIYFILTLFWGSFFGSIFMLSPFLPLMFVNPSWYRWINNRLVATWLTLPVALLETMFGVKVIITGDAFVPGERSVIIMNHRTRMDWMFLWNCLMRYSYLRLEKICLKASLKGVPGFGWAMQAAAYIFIHRKWKDDKSHFEDMIDYFCDIHEPLQLLIFPEGTDLTENSKSRSNAFAEKNGLQKYEYVLHPRTTGFTFVVDRLREGKNLDAVHDITVAYPHNIPQSEKHLLQGDFPREIHFHVHRYPIDTLPTSKEDLQLWCHKRWEEKEERLRSFYQGEKNFYFTGQSVIPPCKSELRVLVVKLLSILYWTLFSPAMCLLIYLYSLVKWYFIITIVIFVLQERIFGGLEIIELACYRLLHKQPHLNSKKNE

分子量

55.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LCLAT1 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LCLAT1 Protein, Human (Cell-Free, His)
目录号:
HY-P702357
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