LILRB4/CD85k/ILT3 蛋白, Human (237a.a, HEK293, Fc)

LILRB4 is a leukocyte immunoglobulin-like receptor located on chromosome 19q13.4 and has an extracellular immunoglobulin domain, a transmembrane domain and a cytoplasmic ITIM. It is expressed on immune cells, interacts with class I MHC, and inhibits the stimulation of immune responses. LILRB4/CD85k/ILT3 Protein, Human (237a.a, HEK293, Fc) is the recombinant human-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LILRB4/CD85k/ILT3 Protein, Human (237a.a, HEK293, Fc) is 237 a.a., with molecular weight of 60-75 kDa.

LILRB4 是白细胞免疫球蛋白样受体 (LIR) 家族的成员，位于染色体区域 19q13.4 的基因簇中。它属于 LIR 受体 B 亚家族，包含多个细胞外免疫球蛋白结构域、一个跨膜结构域和基于细胞质免疫受体酪氨酸的抑制基序 (ITIM)。这种受体表达在免疫细胞上，与抗原呈递细胞上的 MHC I 类分子相互作用，触发抑制免疫反应刺激的负信号。此外，LILRB4 在抗原捕获和呈递中发挥作用，可能影响炎症反应和细胞毒性，从而调节免疫反应并防止自身反应。该基因在多种组织中广泛表达，包括阑尾 (RPKM 9.2)、淋巴结 (RPKM 8.1) 和 17 个其他组织。已鉴定出该基因编码不同亚型的多个转录变体。

1.Immobilized Human ILT3, at 1 μg/mL (100 μL/well) can bind Anti-ILT3 Antibody. The ED₅₀ is 14.79 ng/mL, corresponding to a specific activity is 6.76×10⁴ U/mg.
2.Immobilized Human LILRB4 at 1 μg/mL (100 μL/well) can bind Anti-LILRB4 Antibody. The ED₅₀ for this effect is 9.525 ng/mL.
3.Immobilized Human LILRB4, hFc Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC₅₀ of ≤22.3 ng/mL determined by ELISA.

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  Immobilized Human ILT3, at 1μg/mL (100 μL/well) can bind Anti- ILT3 Antibody, The ED₅₀ for this effect is 14.79 ng/mL，corresponding to a specific activity is 6.76×10⁴ U/mg.

Human

HEK293

C-hFc

NP_001265356.2 (M21-H257)

11006  [NCBI]

MQAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEGPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRH

60-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.