Lipocalin-2/NGAL 蛋白, Human (HEK293, His)

Lipocalin-2/NGAL is an iron transporter that plays a crucial role in various cellular processes. It interacts with the siderophore 2,3-DHBA to transport iron into or out of cells depending on cellular needs. Lipocalin-2/NGAL Protein, Human (HEK293, His) is the recombinant human-derived Lipocalin-2/NGAL protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Lipocalin-2/NGAL Protein, Human (HEK293, His) is 178 a.a., with molecular weight of ~21-24 kDa.

Lipocalin-2/NGAL 是一种铁转运蛋白，在多种细胞过程中发挥着关键作用，包括细胞凋亡、先天免疫和肾脏发育。通过与铁载体 2,3-二羟基苯甲酸 (2,3-DHBA) 的相互作用（结构与细菌肠杆菌素相似），Lipocalin-2/NGAL 可作为铁转运入或转运出细胞的多功能介质，具体取决于特定的细胞要求。铁结合形式 (holo-24p3) 在与 SLC22A17 (24p3R) 受体结合后发生内化，导致铁的释放并随后导致细胞内铁浓度升高。相反，无铁形式 (apo-24p3) 与 SLC22A17 (24p3R) 受体的结合启动涉及细胞内铁载体的级联反应，导致铁螯合和随后的细胞外铁转移，最终降低细胞内铁水平。在白介素 3 (IL3) 剥夺诱导细胞凋亡的情况下，铁负载形式会增加细胞内铁浓度而不引发细胞凋亡，而无铁形式会降低细胞内铁水平，诱导促凋亡蛋白 BCL2L11/BIM 的表达，从而促进细胞凋亡。Lipocalin-2/NGAL 参与先天免疫表现为它通过隔离与微生物铁载体（如肠杆菌素）结合的铁来限制细菌增殖。此外，Lipocalin-2/NGAL 具有结合结核分枝杆菌铁载体的能力。其结构排列包括单体状态和通过二硫键连接的同二聚体，以及与 MMP9 结合的异二聚体形式。

Fully biologically active determined by the dose dependent decrease in SH-SY5Y cell number. The ED₅₀ this effect is 0.1265 μg/mL, corresponding to a specific activity is 7.91×10³ units/mg.

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  Fully biologically active determined by the dose dependent decrease in SH-SY5Y cell number.  The ED₅₀ for this effect is 0.1265 μg/mL, corresponding to a specific activity is 7.91×10³ units/mg.

Human

HEK293

C-6*His

P80188 (Q21-G198)

3934  [NCBI]

QDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG

Approximately 21-24 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.