2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. MARCHF5 蛋白, Mouse (Cell-Free, His, HiBiT)

MARCHF5 蛋白, Mouse (Cell-Free, His, HiBiT)

目录号: HY-P702367
产品使用指南

MARCHF5 蛋白是一种线粒体 E3 泛素蛋白连接酶，可主动调节线粒体裂变并影响线粒体形态。此外，它在防止细胞衰老和帮助线粒体质量控制方面发挥着关键作用。MARCHF5 蛋白, Mouse (Cell-Free, His, HiBiT) 是重组的 MARCHF5 蛋白，由 E. coli Cell-free 表达，带有 N-HiBiT, N-8*His 标签。MARCHF5 蛋白, Mouse (Cell-Free, His, HiBiT) 全长 278 个氨基酸，分子量为 35.6 kDa。

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

MARCHF5 protein is a mitochondrial E3 ubiquitin protein ligase that actively regulates mitochondrial fission and affects mitochondrial morphology. Additionally, it plays a key role in preventing cellular senescence and aids in mitochondrial quality control. MARCHF5 Protein, Mouse (Cell-Free, His, HiBiT) is the recombinant mouse-derived MARCHF5 protein, expressed by E. coli Cell-free , with N-HiBiT, N-8*His labeled tag. The total length of MARCHF5 Protein, Mouse (Cell-Free, His, HiBiT) is 278 a.a., with molecular weight of 35.6 kDa.
研究背景

MARCHF5 蛋白作为线粒体 E3 泛素蛋白连接酶，通过正向调节线粒体裂变对线粒体形态产生显着影响。除了在塑造线粒体结构方面的作用外，MARCHF5 还参与预防细胞衰老，在线粒体质量控制中发挥着关键作用。其活性涉及参与线粒体动力学的关键蛋白的泛素化，即 FIS1、DNM1L 和 MFN1。通过这些调控作用，MARCHF5 成为维持线粒体完整性和细胞稳态的多方面贡献者。
种属

Mouse
表达系统

E. coli Cell-free
标签

N-HiBiT;N-8*His
蛋白编号

Q3KNM2 (M1-A278)
基因 ID

/
蛋白结构
N-term
8*His-HiBiT
MARCHF5 (M1-A278)
Accession # Q3KNM2
C-term
同用名
E3 ubiquitin-protein ligase MARCHF5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; MARCH-V; RING-type E3 ubiquitin transferase MARCHF5
氨基酸序列

MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA

分子量

35.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

MARCHF5 Protein, Mouse (Cell-Free, His, HiBiT) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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