NCKIPSD 蛋白, Human (His)

NCKIPSD Protein, vital for stress fiber formation induced by active DRF1, stimulates N-WASP-induced ARP2/3 complex activation, independent of CDC42. Implicated in sarcomere maintenance, myofibril assembly, actin polymerization, and angiogenesis. Associates with vimentin, desmin, NCK, GRB2, and interacts with Helicobacter pylori toxin vacA, FHOD1, FASLG, and TMIGD2, showcasing its diverse cellular interactions. NCKIPSD Protein, Human (His) is the recombinant human-derived NCKIPSD protein, expressed by E. coli, with N-His labeled tag. The total length of NCKIPSD Protein, Human (His) is 244 a.a., with molecular weight of ~33-45 kDa.

NCKIPSD 蛋白在活性透明蛋白同源物 1 (DRF1) 诱导的应力纤维的形成中发挥着至关重要的作用，并促进体内微刺的形成。在体外没有 CDC42 的情况下，它还能刺激 N-WASP 诱导的 ARP2/3 复合物激活。此外，它还参与肌节的维护和肌原纤维组装成肌节，有助于肌动蛋白聚合和细胞粘附调节。NCKIPSD 蛋白参与血管生成并与波形蛋白和结蛋白等中间丝相关。它与 NCK、GRB2 和透明蛋白同源物 1 (DRF1) 的 SH3 结构域以及 N-WASP 富含脯氨酸的结构域结合。它与幽门螺杆菌毒素 vacA、FHOD1（亚型 4）、FASLG 和 TMIGD2 相互作用。

Human

E. coli

N-His

Q9NZQ3-3 (M1-T244)

51517  [NCBI]

MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGT

Approximately 33-45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.