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NKG7 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702386
产品使用指南

NKG7 蛋白是细胞毒性颗粒胞吐作用的重要调节因子,介导感染性和非感染性疾病中的炎症。其重要作用包括促进NK细胞和CD8+T细胞的细胞毒性脱颗粒、激活CD4+T细胞以及通过穿孔素/颗粒酶途径增强细胞溶解活性。NKG7 蛋白, Mouse (Cell-Free, His) 是重组的 NKG7 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。NKG7 蛋白, Mouse (Cell-Free, His) 全长 165 个氨基酸,分子量为 19.5 kDa。

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描述

The NKG7 protein is an important regulator of cytotoxic granule exocytosis and mediates inflammation in infectious and non-infectious diseases. Its important roles include promoting cytotoxic degranulation of NK cells and CD8(+) T cells, activating CD4(+) T cells, and enhancing cytolytic activity through the perforin/granzyme pathway. NKG7 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived NKG7 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of NKG7 Protein, Mouse (Cell-Free, His) is 165 a.a., with molecular weight of 19.5 kDa.

研究背景

NKG7 蛋白作为效应淋巴细胞中细胞毒性颗粒胞吐作用的关键调节因子,成为多种感染性和非感染性疾病中炎症的关键介质。它在促进自然杀伤(NK)细胞和CD8(+)T细胞的细胞毒性脱颗粒以及激活CD4(+)T细胞以应对感染方面发挥着明显的不可或缺的作用。值得注意的是,NKG7 在 CD8+ T 细胞和 NK 细胞对靶细胞的细胞溶解中发挥着关键作用,通过穿孔素/颗粒酶途径增强细胞溶解活性,特别是通过促进携带 LAMP1 的溶解颗粒的胞吐作用。此外,NKG7 对 NK 细胞介导的癌症转移控制的贡献强调了其在免疫监视和针对恶性细胞的反应中的更广泛影响。

种属

Mouse

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

Q99PA5 (M1-L165)

基因 ID

/

蛋白结构
N-term
10*His
NKG7 (M1-L165)
Accession # Q99PA5
C-term
同用名
Protein NKG7; Natural killer cell protein 7
氨基酸序列

MEPCRSLALFAGSLGLTSSLIALTTDFWIVATGPHFSAHSGLWPTSQETQVAGYIHVTQSFCILAVLWGLVSVSFLILSCIPALSAPGRGPLVSTVMAFSAALSILVAMAVYTSMRWSQTPFSQVQTFFSWSFYLGWVSFILFLFAGCLSLGAHCRTRRAEYETL

分子量

19.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NKG7 Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NKG7 Protein, Mouse (Cell-Free, His)
目录号:
HY-P702386
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