NT5C1A 蛋白, Human (P.pastoris, His)

The NT5C1A protein plays a key role in cellular nucleotide metabolism, catalyzing the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates and releasing inorganic phosphates and the corresponding nucleosides. Although AMP was identified as the primary substrate of NT5C1A, this versatile enzyme is also capable of hydrolyzing other nucleotides, such as dCMP and IMP. NT5C1A Protein, Human (P.pastoris, His) is the recombinant human-derived NT5C1A protein, expressed by P. pastoris , with N-His labeled tag. The total length of NT5C1A Protein, Human (P.pastoris, His) is 368 a.a., with molecular weight of ~43.0 kDa.

NT5C1A，也称为胞质嘌呤5'-核苷酸酶，是一种催化核糖核苷酸和脱氧核糖核苷酸单磷酸水解的酶。在此过程中，释放出无机磷酸盐和相应的核苷。虽然单磷酸腺苷 (AMP) 是主要底物，但 NT5C1A 还可以水解其他核苷酸，包括单磷酸脱氧胞苷 (dCMP) 和单磷酸肌苷 (IMP)。这种酶活性对于核苷酸库的调节至关重要，通过控制核苷单磷酸的水平有助于维持细胞稳态。它必须强调 NT5C1A 在水解不同底物方面的多功能性，强调其在核苷酸代谢中的作用。

Human

P. pastoris

N-His

Q9BXI3 (1M-368Q)

84618  [NCBI]

MEPGQPREPQEPREPGPGAETAAAPVWEEAKIFYDNLAPKKKPKSPKPQNAVTIAVSSRALFRMDEEQQIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNRRLRELYPDSEDVFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADAEKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQTPRRTAPAKQAPSAQ

Approximately 43.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.