2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. O-acyltransferase 蛋白, Tropaeolum majus (Cell-Free, S197A, His)

O-acyltransferase 蛋白, Tropaeolum majus (Cell-Free, S197A, His)

目录号: HY-P702390
产品使用指南

O-酰基转移酶蛋白是甘油脂代谢的核心参与者，特别关注三酰基甘油的生物合成。作为一种酶，它催化酰基转移到甘油脂途径中的各种分子，并在三酰甘油的合成中起着至关重要的作用，而三酰甘油是脂质代谢的重要组成部分。O-acyltransferase 蛋白, Tropaeolum majus (Cell-Free, S197A, His) 是重组的 O-酰基转移酶蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。O-acyltransferase 蛋白, Tropaeolum majus (Cell-Free, S197A, His) 全长 518 个氨基酸，分子量为 60.3 kDa。

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描述

O-acyltransferase proteins are central players in glycerolipid metabolism, with a particular focus on triacylglycerol biosynthesis. As an enzyme, it catalyzes the transfer of acyl groups to various molecules in the glycerolipid pathway and plays a crucial role in the synthesis of triacylglycerol, which is an important component of lipid metabolism. O-acyltransferase Protein, Tropaeolum majus (Cell-Free, S197A, His) is the recombinant O-acyltransferase protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of O-acyltransferase Protein, Tropaeolum majus (Cell-Free, S197A, His) is 518 a.a., with molecular weight of 60.3 kDa.
研究背景

O-酰基转移酶蛋白与甘油脂代谢有关，特别是在三酰甘油生物合成（脂质代谢中的一个重要过程）中发挥着至关重要的作用。三酰甘油是参与能量储存的重要分子，是细胞中脂滴的关键成分。O-酰基转移酶是负责催化酰基转移反应的酶，酰基转移反应有助于三酰基甘油的形成。通过参与这些生物合成途径，O-酰基转移酶有助于调节细胞内的脂质稳态和能量储存。
种属

Others
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

Q8RX96 (M1-K518, S197A)
基因 ID

/
蛋白结构
N-term
10*His
O-acyltransferase (M1-K518, S197A)
Accession # Q8RX96
C-term
同用名
O-acyltransferase
氨基酸序列

MAVAESSQNTTTMSGHGDSDLNNFRRRKPSSSVIEPSSSGFTSTNGVPATGHVAENRDQDRVGAMENATGSVNLIGNGGGVVIGNEEKQVGETDIRFTYRPSFPAHRRVRESPLSSDAIFKQSHAGLFNLCIVVLIAVNSRLIIENLMKYGWLIDTGFWFSSRSLGDWSIFMCCLTLPIFPLAAFIVEKLVQRNHIAELVAVLLHVIVSTAAVLYPVIVILTCDSVYMSGVVLMLFGCIMWLKLVSYAHTSSDIRTLAKSGYKGDAHPNSTIVSCSYDVSLKSLAYFMVAPTLCYQPSYPRSSCIRKGWVVRQFVKLIVFIGLMGFIIEQYINPIVRNSKHPLKGDFLYAIERVLKLSVPNLYVWLCMFYSFFHLWLNILAELLRFGDREFYKDWWNAKTVAEYWKMWNMPVHRWMVRHLYFPCLRNGIPKEGAIIIAFLVSGAFHELCIAVPCHVFKLWAFIGIMFQVPLVLITNYLQEKFSNSMVGNMIFWFIFCILGQPMCVLLYYHDLINLKEK

分子量

60.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

O-acyltransferase Protein, Tropaeolum majus (Cell-Free, S197A, His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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