PAH 蛋白, Human (D415N, sf9, His)

PAH protein is a key enzyme involved in amino acid metabolism. Its main function is to catalyze the hydroxylation of L-phenylalanine and convert it into L-tyrosine. PAH Protein, Human (D415N, sf9, His) is the recombinant human-derived PAH protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PAH Protein, Human (D415N, sf9, His) is 452 a.a., with molecular weight of ~52 kDa.

PAH蛋白是参与氨基酸代谢的关键酶。其主要功能是催化 L-苯丙氨酸的羟基化，将其转化为 L-酪氨酸。这种酶活性是将一种氨基酸转化为另一种氨基酸的关键步骤，特别有助于从苯丙氨酸合成酪氨酸。这个过程对于需要产生酪氨酸的各种生物过程至关重要，酪氨酸是合成神经递质、甲状腺激素和黑色素等重要分子的基础。PAH蛋白的活性对于维持适当的氨基酸平衡和支持体内的各种生理功能起着关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P00439 (M1-K452,D415N)

5053  [NCBI]

MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYNPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK

Approximately 52 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.