PCNA 蛋白, Human (sf9, His)

PCNA, an atypical member of the sulfotransferase family, displays notably low affinity for 3'-phospho-5'-adenylyl sulfate (PAPS) and minimal catalytic activity towards substrates like L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphthylamine, and 2-beta-naphthol. Its precise function remains elusive, but PCNA is suggested to participate in drug and neurotransmitter metabolism within the central nervous system (CNS). PCNA Protein, Human (sf9, His) is the recombinant human-derived PCNA protein, expressed by Sf9 insect cells, with N-His labeled tag. The total length of PCNA Protein, Human (sf9, His) is 261 a.a., with molecular weight of ~36 kDa.

SULT4A1 是磺基转移酶家族的非典型成员，对 3'-磷酸-5'-腺苷基硫酸盐 (PAPS) 表现出明显低的亲和力，并对各种底物表现出最低的催化活性，包括 L-三碘甲状腺原氨酸、甲状腺素、雌酮、对硝基苯酚、 2-萘胺和2-β-萘酚。虽然其精确功能仍难以捉摸，但 SULT4A1 被认为在中枢神经系统 (CNS) 内的药物和神经递质代谢中发挥作用。

Human

Sf9 insect cells

N-His

P12004 (M1-S261)

5111  [NCBI]

MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS

Approximately 36 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Na₃PO₄, 300 mM NaCl, 10% Glycerol, pH 7.0, 2 mM DTT.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.