PIN1 蛋白, Human (His)

The PIN1 protein is a peptidyl-prolyl cis/trans isomerase that complexly regulates multiple cellular processes by binding and isomerizing phosphorylated Ser/Thr-Pro motifs. This molecular switch induces conformational changes in phosphorylated proteins that affect mitosis, kinase activity, oncogene transactivation, centrosome amplification, and cell transformation. PIN1 Protein, Human (His) is the recombinant human-derived PIN1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PIN1 Protein, Human (His) is 163 a.a., with molecular weight of ~19 kDa.

PIN1 蛋白是一种肽基脯氨酰顺/反异构酶 (PPIase)，通过结合特定磷酸化 Ser/Thr-Pro (pSer/Thr-Pro) 基序并使其异构化，复杂地参与多种细胞过程。这种分子开关诱导磷酸化蛋白质的构象变化，影响多种细胞途径。由于偏爱脯氨酸键 N 端的酸性残基，PIN1 通过与 NIMA 相互作用来调节有丝分裂，减弱其有丝分裂促进活性，并下调 BTK 的激酶活性。它反式激活癌基因，诱导中心体扩增、染色体不稳定和细胞转化。此外，PIN1 对于 RAF1 的去磷酸化和回收、结合并靶向 PML 和 BCL6 进行降解以及通过破坏 FBXW7 二聚化作为 JNK 级联的调节器至关重要。PIN1 的参与延伸到 DNA 修复过程，影响易错非同源末端连接 (NHEJ) 和无错同源重组 (HR) 之间的平衡。在 IL33 诱导的肺部炎症过程中，PIN1 催化磷酸化 IRAK3/IRAK-M 的顺反异构化，有助于 IRAK3 稳定、核易位以及树突状细胞中促炎基因的表达。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q13526 (M1-E163)

5300  [NCBI]

MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE

Approximately19 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.