PSAP/Prosaposin 蛋白, Human (HEK293, His)

Frizzled-7 protein is a transmembrane receptor that plays a crucial role in the Wnt signaling pathway. It is involved in cellular processes such as cell proliferation, differentiation, and migration. PSAP/Prosaposin Protein, Human (HEK293, His) is the recombinant human-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-His labeled tag. The total length of PSAP/Prosaposin Protein, Human (HEK293, His) is 508 a.a., with molecular weight of 60-85 kDa.

该基因编码的 PSAP/Prosaposin 蛋白是一种高度保守的前蛋白原，经过蛋白水解加工产生四种主要裂解产物 - saposin A、B、C 和 D。前体蛋白的每个结构域长约 80 个氨基酸残基，表现出几乎相同的半胱氨酸残基和糖基化位点的位置。Saposins AD 主要定位于溶酶体区室，在促进具有短寡糖基团的鞘糖脂的分解代谢中发挥着至关重要的作用。前体蛋白以分泌型和整合膜型两种形式存在，并具有神经营养活性。该基因的突变与戈谢病和异染性脑白质营养不良有关。选择性剪接产生多种转录变体，其中至少一种编码经过蛋白水解加工的同工型。该基因表现出无处不在的表达，在脾脏 (RPKM 523.6)、胆囊 (RPKM 491.5) 和其他 25 个组织中检测到表达水平升高，强调了其在多个器官的各种生理环境中的重要作用。

Data is not available.

Human

HEK293

C-His

NP_002769.1 (G17-N524)

5660  [NCBI]

GPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQCNAVEHCKRHVWN

60-85 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.