1. 重组蛋白
  2. Enzymes & Regulators
  3. Carboxypeptidase
  4. PSMA 蛋白, Human (HEK293, His, solution)

PSMA 蛋白, Human (HEK293, His, solution)

目录号: HY-P70548
COA 产品使用指南 技术支持

PSMA 蛋白 是一种高度表达于前列腺癌细胞表面的 II 型跨膜糖蛋白。PSMA 蛋白 通过叶酸水解酶活性将细胞外多聚谷氨酸叶酸水解为单谷氨酸叶酸,提升肿瘤细胞对叶酸的摄取效率以支持增殖;通过 NAALADase 活性参与神经肽代谢。PSMA 蛋白, Human (HEK293, His, solution) 是重组的 PSMA 蛋白,由 HEK293 表达,带有 N-6*His 标签。

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规格 价格 是否有货 数量
10 μg ¥1700 In-stock
50 μg ¥4800 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PSMA Protein is a type II transmembrane glycoprotein highly expressed on the surface of prostate cancer cells. PSMA Protein hydrolyzes extracellular polyglutamic acid folate into monoglutamic acid folate through folate hydrolase activity, improving the uptake efficiency of folate by tumor cells to support proliferation; and participates in neuropeptide metabolism through NAALADase activity. PSMA Protein, Human (HEK293, His, solution) is a recombinant PSMA protein expressed by HEK293 with an N-6*His tag.

研究背景

PSMA Protein 属于肽酶 M28 家族 (M28 peptidase family) [1]PSMA Protein 是一种高度表达于前列腺癌细胞表面的 II 型跨膜糖蛋白[1][2] [5]PSMA Protein 通过叶酸水解酶活性将细胞外多聚谷氨酸叶酸水解为单谷氨酸叶酸,提升肿瘤细胞对叶酸的摄取效率以支持增殖;通过 NAALADase 活性参与神经肽代谢;其表达可能通过促进整合素信号传导介导肿瘤血管生成[1][5]PSMA Protein 在正常前列腺上皮细胞、肾脏近曲小管、中枢神经系统、小肠黏膜细胞及唾液腺中低水平表达,在前列腺癌细胞及多种实体瘤的新生血管内皮细胞中显著高表达[1][2][5]PSMA Protein, Human 由 750 个氨基酸残基构成,包含 19 个氨基酸的胞内结构域、24 个氨基酸的跨膜结构域和 707 个氨基酸的胞外结构域,胞外域含有 10 个 N-糖基化位点,糖基化修饰影响其稳定性和功能[1][4]

体外研究
(In Vitro)

PSMA Protein, Human (转染 pLNCX-PSMA 质粒到 PC-3 细胞) 显著促进 PC-3 细胞增殖[1]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

N-6*His

蛋白编号

Q04609-1 (K44-A750)

基因 ID
蛋白结构
N-term
6*His
PSMA (K44-A750)
Accession # Q04609-1
C-term
蛋白长度

Extracellular Domain

中文名
PSMA 蛋白
同用名
Glutamate carboxypeptidase 2; FGCP; GCPII; mGCP; NAALADase I; PSMA; Cell growth-inhibiting gene 27 protein; Folate hydrolase 1
氨基酸序列

KSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA

分子量

90-120 kDa

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 5% Trehalose, pH 5.5.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

PSMA Protein, Human (HEK293, His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PSMA Protein, Human (HEK293, His, solution)
目录号:
HY-P70548
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