PSMA 蛋白, Human (HEK293, His, solution)

PSMA Protein is a type II transmembrane glycoprotein highly expressed on the surface of prostate cancer cells. PSMA Protein hydrolyzes extracellular polyglutamic acid folate into monoglutamic acid folate through folate hydrolase activity, improving the uptake efficiency of folate by tumor cells to support proliferation; and participates in neuropeptide metabolism through NAALADase activity. PSMA Protein, Human (HEK293, His, solution) is a recombinant PSMA protein expressed by HEK293 with an N-6*His tag.

PSMA Protein 属于肽酶 M28 家族 (M28 peptidase family) ^[1]。PSMA Protein 是一种高度表达于前列腺癌细胞表面的 II 型跨膜糖蛋白^{[1][2] [5]}。PSMA Protein 通过叶酸水解酶活性将细胞外多聚谷氨酸叶酸水解为单谷氨酸叶酸，提升肿瘤细胞对叶酸的摄取效率以支持增殖；通过 NAALADase 活性参与神经肽代谢；其表达可能通过促进整合素信号传导介导肿瘤血管生成^[1][5]。PSMA Protein 在正常前列腺上皮细胞、肾脏近曲小管、中枢神经系统、小肠黏膜细胞及唾液腺中低水平表达，在前列腺癌细胞及多种实体瘤的新生血管内皮细胞中显著高表达^[1][2][5]。PSMA Protein, Human 由 750 个氨基酸残基构成，包含 19 个氨基酸的胞内结构域、24 个氨基酸的跨膜结构域和 707 个氨基酸的胞外结构域，胞外域含有 10 个 N-糖基化位点，糖基化修饰影响其稳定性和功能^[1][4]。

PSMA Protein, Human (转染 pLNCX-PSMA 质粒到 PC-3 细胞) 显著促进 PC-3 细胞增殖^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

N-6*His

Q04609-1 (K44-A750)

2346  [NCBI]

Extracellular Domain

KSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA

90-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 5% Trehalose, pH 5.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Yao V, et al. Expression of prostate-specific membrane antigen (PSMA), increases cell folate uptake and proliferation and suggests a novel role for PSMA in the uptake of the non-polyglutamated folate, folic acid. Prostate. 2010 Feb 15;70(3):305-16.  [Content Brief]

  [2]. Kranzbühler B, et al. Pharmacological upregulation of prostate-specific membrane antigen (PSMA) expression in prostate cancer cells. Prostate. 2018 Jul;78(10):758-765.  [Content Brief]

  [3]. Hrkach J, et al. Preclinical development and clinical translation of a PSMA-targeted docetaxel nanoparticle with a differentiated pharmacological profile. Sci Transl Med. 2012 Apr 4;4(128):128ra39.  [Content Brief]

  [4]. Yuan W, et al. Glycoforms of human prostate-specific membrane antigen (PSMA) in human cells and prostate tissue. Prostate. 2022 Jan;82(1):132-144.  [Content Brief]

  [5]. Holmes EH. PSMA specific antibodies and their diagnostic and therapeutic use. Expert Opin Investig Drugs. 2001 Mar;10(3):511-9.  [Content Brief]