RGMA 蛋白, Human (His)

Repulsive guidance molecule A (RGMA) is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. RGMA regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA also functions as a bone morphogenetic protein (BMP) coreceptor and may act as a tumor suppressor in some cancers. RGMA Protein, Human (His) is the recombinant human-derived RGMA protein, expressed by E. coli , with N-6*His labeled tag. The total length of RGMA Protein, Human (His) is 254 a.a., with molecular weight of ~30.0 kDa.

排斥引导分子 A (RGMA) 是排斥引导分子家族中的一员。RGMA 是一种糖基磷脂酰肌醇锚定糖蛋白，在发育和成人中枢神经系统中起轴突引导蛋白的作用。RGMA 调节头侧神经管闭合，抑制神经突生长和皮层神经元分支，以及成熟突触的形成。RGMA 通过与受体 NEO1/neogenin 结合，通过UNC5B-ARHGEF12/LARG-PTK2/FAK1 级联诱导 RHOA-ROCK1/Rho 激酶信号通路的激活，导致神经元生长锥塌陷和神经突生长抑制。NEO1/neogenin 与 RGMA 的结合还通过影响 HRAS-PTK2/FAK1-AKT1 通路导致 HRAS 失活。RGMA 还作为骨形态发生蛋白 (BMP) 的辅助受体，可能通过 SMAD1、SMAD5 和 SMAD8 发出信号。RGMA 还可能在某些癌症中起肿瘤抑制作用^[1][2][3]。

Human

E. coli

N-6*His

AAI51133.1 (P169-G422)

56963  [NCBI]

PHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.