1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. Sensor histidine kinase NatK 蛋白, Bacillus subtilis (Cell-Free, His)

Sensor histidine kinase NatK 蛋白, Bacillus subtilis (Cell-Free, His)

目录号: HY-P702431
产品使用指南

传感器组氨酸激酶 NatK 蛋白是 NatK/NatR 系统组件,可正向调节 natAB 操纵子的表达。它可以磷酸化 NatR,介导细菌细胞中的信号转导。NatK/NatR 系统例证了调节基因表达的分子机制,强调了细菌信号通路中传感器组氨酸激酶精心策划的复杂调节过程。Sensor histidine kinase NatK 蛋白, Bacillus subtilis (Cell-Free, His) 是重组的 传感器组氨酸激酶 NatK 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。Sensor histidine kinase NatK 蛋白, Bacillus subtilis (Cell-Free, His) 全长 318 个氨基酸,分子量为 36.6 kDa。

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描述

Sensor histidine kinase NatK Protein, a NatK/NatR system component, positively regulates natAB operon expression. It can phosphorylate NatR, mediating signal transduction in bacterial cells. The NatK/NatR system exemplifies a molecular mechanism for modulating gene expression, emphasizing the intricate regulatory processes orchestrated by sensor histidine kinases in bacterial signaling pathways. Sensor histidine kinase NatK Protein, Bacillus subtilis (Cell-Free, His) is the recombinant Sensor histidine kinase NatK protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Sensor histidine kinase NatK Protein, Bacillus subtilis (Cell-Free, His) is 318 a.a., with molecular weight of 36.6 kDa.

研究背景

传感器组氨酸激酶 NatK 蛋白是二元调节系统 NatK/NatR 的一个组成部分,在控制 natAB 操纵子表达中充当正调节因子。该蛋白具有磷酸化 NatR 的潜力,突出了其在介导细菌细胞内信号转导中的作用。NatK/NatR 系统代表细胞用来调节基因表达的分子机制,强调细菌信号通路中传感器组氨酸激酶精心策划的复杂调节过程。

种属

Others

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P70954 (M1-K318)

基因 ID

938382

蛋白结构
N-term
10*His
NatK (M1-K318)
Accession # P70954
C-term
同用名
Sensor histidine kinase NatK
氨基酸序列

MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSAQPKADTQAYIQNWADQYSQYDRFLKGENAYVAGVLYDFLEKARASNVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKNGAHEGMGTYIIQKLVKGAFGRLDFTYRHPIFRLEIKIPFQK

分子量

36.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Sensor histidine kinase NatK Protein, Bacillus subtilis (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Sensor histidine kinase NatK Protein, Bacillus subtilis (Cell-Free, His)
目录号:
HY-P702431
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