SGMS1 蛋白, Human (Cell-Free, His)

The SGMS1 protein is a key sphingomyelin synthase located in the Golgi apparatus and promotes sphingomyelin biosynthesis by transferring the phosphocholine moiety. This reversible reaction involves the conversion of phosphatidylcholine and ceramide into sphingomyelin and diacylglycerol. SGMS1 Protein, Human (Cell-Free, His) is the recombinant human-derived SGMS1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of SGMS1 Protein, Human (Cell-Free, His) is 413 a.a., with molecular weight of 51.4 kDa.

SGMS1 蛋白作为高尔基体的主要鞘磷脂合酶，通过可逆磷酸胆碱转移协调鞘磷脂生物合成。在正向反应中，它将磷酸胆碱头基从磷脂酰胆碱转移到神经酰胺，形成鞘磷脂和二酰甘油。相反，在逆反应中，SGMS1 将磷酸胆碱从鞘磷脂转移到二酰甘油，生成磷脂酰胆碱和神经酰胺。方向性取决于高尔基膜神经酰胺和二酰甘油的水平。值得注意的是，SGMS1 不利用游离磷酸胆碱或 CDP-胆碱作为供体。在功能上，它通过促有丝分裂的二酰甘油和促凋亡神经酰胺调节受体介导的信号转导。此外，它还通过调节高尔基体上的 DAG 库来影响分泌转运，影响下游 PRKD1 并有助于信号转导和蛋白质分选的膜筏形成。

Human

E. coli Cell-free

N-10*His

Q86VZ5 (M1-T413)

259230

MKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLCRVSSDNGQRLLDMIETLKMEHHLEAHKNGHANGHLNIGVDIPTPDGSFSIKIKPNGMPNGYRKEMIKIPMPELERSQYPMEWGKTFLAFLYALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLIQWLLLKYKSIISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQLRRIMKLIAGGGLSITGSHNMCGDYLYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLFWWYHTMANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT

51.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.