STING1 蛋白, Mouse (Cell-Free, His)

STING1 is a promoter of innate immune signaling, a key sensor of bacterial and viral cytoplasmic DNA, and promotes the production of type I interferons (IFN-α and IFN-β). This innate immune response is triggered in response to non-CpG double-stranded DNA delivered to the cytoplasm from various pathogens. STING1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived STING1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of STING1 Protein, Mouse (Cell-Free, His) is 378 a.a., with molecular weight of 48.9 kDa.

STING1 是先天免疫信号传导的促进剂，是细菌和病毒胞质 DNA 的关键传感器，促进 I 型干扰素（IFN-α 和 IFN-β）的产生。这种先天免疫反应是响应从各种病原体传递到细胞质的非 CpG 双链 DNA 而触发的。STING1 通过结合环状二核苷酸、识别并结合细菌第二信使环二 GMP (c-di-GMP) 和响应细胞质中 DNA 病毒而产生的环 GMP-AMP (cGAMP) 来发挥作用。结合后，STING1 寡聚化，从内质网移位，并被 TBK1 磷酸化，导致转录因子 IRF3 的募集和激活。这种诱导导致 I 型干扰素的表达，建立有效的抗病毒状态。STING1 的多功能作用延伸到直接参与自噬，其中 cGAMP 结合触发其从内质网移动到 COPII 囊泡，形成内质网-高尔基中间室 (ERGIC)。ERGIC 作为自噬体形成的膜源，促进溶酶体降解胞质 DNA 或 DNA 病毒。STING1 的自噬和干扰素诱导活性可以分离，自噬诱导与 TBK1 磷酸化无关。此外，STING1 对 2'-3'-cGAMP 表现出偏好，并可能参与易位子功能，影响 I 型干扰素的诱导。此外，它与主要组织相容性复合物 II 类的关联表明其在转导细胞凋亡信号中具有潜在作用。STING1 被抗癌药物 5,6-二甲基呫吨酮 4-乙酸 (DMXAA) 激活，并被硝基呋喃衍生物 C-178 和 C-176 特异性抑制，从而防止 STING1 的棕榈酰化和随后的激活。STING1 的多方面活性凸显了其在协调先天免疫反应、自噬过程以及对细胞凋亡信号传导和抗癌反应的潜在贡献方面的关键作用。

Mouse

E. coli Cell-free

N-10*His

Q3TBT3 (M1-I378)

72512

MPYSNLHPAIPRPRGHRSKYVALIFLVASLMILWVAKDPPNHTLKYLALHLASHELGLLLKNLCCLAEELCHVQSRYQGSYWKAVRACLGCPIHCMAMILLSSYFYFLQNTADIYLSWMFGLLVLYKSLSMLLGLQSLTPAEVSAVCEEKKLNVAHGLAWSYYIGYLRLILPGLQARIRMFNQLHNNMLSGAGSRRLYILFPLDCGVPDNLSVVDPNIRFRDMLPQQNIDRAGIKNRVYSNSVYEILENGQPAGVCILEYATPLQTLFAMSQDAKAGFSREDRLEQAKLFCRTLEEILEDVPESRNNCRLIVYQEPTDGNSFSLSQEVLRHIRQEEKEEVTMNAPMTSVAPPPSVLSQEPRLLISGMDQPLPLRTDLI

45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.