2. 重组蛋白
  3. Receptor Proteins
  4. TAS2R13 蛋白, Human (Cell-Free, His)

TAS2R13 蛋白, Human (Cell-Free, His)

目录号: HY-P702468
产品使用指南

TAS2R13 蛋白是一种可能与苦味感知有关的受体，与味素相关。它可能有助于感知胃肠内容物的化学成分。该受体的激活会刺激α味觉素，触发PLC-β-2激活，并导致TRPM5的门控，表明其在苦味转导和味觉信号通路中的作用。TAS2R13 蛋白, Human (Cell-Free, His) 是重组的 TAS2R13 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。TAS2R13 蛋白, Human (Cell-Free, His) 全长 303 个氨基酸，分子量为 36.6 kDa。

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描述

TAS2R13 Protein, a receptor possibly involved in bitter taste perception, is linked to gustducin. It likely contributes to sensing the chemical composition of gastrointestinal content. Activation of this receptor stimulates alpha gustducin, triggers PLC-beta-2 activation, and results in the gating of TRPM5, indicating its role in bitter taste transduction and gustatory signaling pathways. TAS2R13 Protein, Human (Cell-Free, His) is the recombinant human-derived TAS2R13 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of TAS2R13 Protein, Human (Cell-Free, His) is 303 a.a., with molecular weight of 36.6 kDa.
研究背景

TAS2R13 作为参与苦味感知的受体发挥作用，并与味素信号通路相关。它很可能在感知胃肠内容物的化学成分方面发挥作用。该受体的激活会刺激 alpha gustducin，促进 PLC-beta-2 的激活，并最终导致 TRPM5 的门控。这种复杂的信号级联表明 TAS2R13 有助于味觉感知的复杂机制，特别是在苦味化合物的检测中，并且可能对胃肠道味觉反应的调节有影响。
种属

Human
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

Q9NYV9 (M1-R303)
基因 ID

50838
蛋白结构
N-term
10*His
TAS2R13 (M1-R303)
Accession # Q9NYV9
C-term
同用名
Taste receptor type 2 member 13; Taste receptor family B member 3; TRB3
氨基酸序列

MESALPSIFTLVIIAEFIIGNLSNGFIVLINCIDWVSKRELSSVDKLLIILAISRIGLIWEILVSWFLALHYLAIFVSGTGLRIMIFSWIVSNHFNLWLATIFSIFYLLKIASFSSPAFLYLKWRVNKVILMILLGTLVFLFLNLIQINMHIKDWLDRYERNTTWNFSMSDFETFSVSVKFTMTMFSLTPFTVAFISFLLLIFSLQKHLQKMQLNYKGHRDPRTKVHTNALKIVISFLLFYASFFLCVLISWISELYQNTVIYMLCETIGVFSPSSHSFLLILGNAKLRQAFLLVAAKVWAKR

分子量

36.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TAS2R13 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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