TCblR/CD320 蛋白, Rat (HEK293, Fc)

The TCblR/CD320 protein is a receptor for cobalamin-saturated transcobalamin (TCbl) and is critical for cobalamin uptake and can influence cell physiology. At the plasma membrane, it is expressed on follicular dendritic cells (FDC), where it helps interact with germinal center B cells and promotes immune responses. TCblR/CD320 Protein, Rat (HEK293, Fc) is the recombinant rat-derived TCblR/CD320 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TCblR/CD320 Protein, Rat (HEK293, Fc) is 182 a.a., with molecular weight of ~47.3 KDa.

TCblR/CD320 蛋白作为钴胺素饱和转钴胺素 (TCbl) 的受体，在钴胺素摄取中发挥着至关重要的作用，证明了其在细胞生理学中的重要性。该受体位于质膜上，主要在滤泡树突细胞 (FDC) 上表达，促进与生发中心 B 细胞的相互作用，并促进复杂的免疫反应网络。TCblR 作为共刺激剂，促进 B 细胞对抗原刺激的反应，从而促进 B 细胞分化和增殖。具体来说，它通过与 T 细胞和 FDC 的协作相互作用，在生发中心 B (GC-B) 细胞分化为记忆 B 细胞和浆细胞 (PC) 过程中发挥着至关重要的作用。值得注意的是，CD320 增强了 IL-10 产生的 PC 前体的增殖，展示了其在免疫调节中的多方面参与。TCblR 与 TCN2 的相互作用进一步凸显了其在钴胺素稳态和细胞过程中的作用。

Measured by its binding ability in a functional ELISA. When Recombinant Rat CD320 is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant mouse TCN2. The ED₅₀ for this effect is 0.1918 μg/mL.

Rat

HEK293

C-hFc

Q5HZW5/NP_001014223.1 (A29-A210)

362851  [NCBI]

APAPTSAPAHTLVQVSGPRAGSCPTDTFKCLTSGYCVPLSWRCDGDRDCSDGSDEEECRIEPCAQNRQCQPQPALPCSCDNISGCSAGSDKNLNCSRSPCQEGELRCILDDVCIPHTWRCDGHPDCPDSSDELSCDTDTETDKIFQEENATTSMSSMIVEKETSFRNVTVASAGHPSRNPNA

Approximately 65-70 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.