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  3. TMEM65 蛋白, Human (Cell-Free, His, SUMO)

TMEM65 蛋白, Human (Cell-Free, His, SUMO)

目录号: HY-P702473
产品使用指南

TMEM65 蛋白对于心脏发育至关重要,可调节 GJA1 并影响间隙连接通讯。它有助于心脏闰盘中 GJA1 的稳定性,并且有迹象表明它参与线粒体功能。TMEM65 蛋白, Human (Cell-Free, His, SUMO) 是重组的 TMEM65 蛋白,由 E. coli Cell-free 表达,带有 N-6*His, N-SUMO 标签。TMEM65 蛋白, Human (Cell-Free, His, SUMO) 全长 178 个氨基酸,分子量为 35.1 kDa。

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描述

The TMEM65 protein is critical for cardiac development, regulates GJA1 and affects gap junction communication. It contributes to the stability of GJA1 in cardiac intercalary disks, and there are indications that it is involved in mitochondrial function. TMEM65 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived TMEM65 protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of TMEM65 Protein, Human (Cell-Free, His, SUMO) is 178 a.a., with molecular weight of 35.1 kDa.

研究背景

TMEM65 蛋白在心脏发育和功能中发挥着潜在的关键作用,对心脏传导的调节和间隙连接蛋白 GJA1 的功能具有影响。通过促进 GJA1 的稳定性和正确定位到心脏闰盘,TMEM65 可能在调节间隙连接通讯中发挥关键作用。此外,有迹象表明 TMEM65 可能参与线粒体呼吸的调节和线粒体 DNA 拷贝数的维持。TMEM65 以单体和同二聚体形式存在,与 GJA1 相互作用,与 DSP 相互作用较弱,表明它多方面参与对心脏和线粒体功能至关重要的细胞过程。进一步的研究对于阐明 TMEM65 发挥其多种功能的复杂分子途径至关重要。

种属

Human

表达系统

E. coli Cell-free

标签

N-6*His;N-SUMO

蛋白编号

Q6PI78 (M63-S240)

基因 ID

157378

蛋白结构
N-term
6*His-SUMO
TMEM65 (M63-S240)
Accession # Q6PI78
C-term
同用名
Transmembrane protein 65
氨基酸序列

MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS

分子量

35.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TMEM65 Protein, Human (Cell-Free, His, SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TMEM65 Protein, Human (Cell-Free, His, SUMO)
目录号:
HY-P702473
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