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  3. TMX1 蛋白, Human (HEK293, Fc)

TMX1 蛋白, Human (HEK293, Fc)

目录号: HY-P76681
产品使用指南

TMX1 蛋白是蛋白二硫键异构酶(PDI) 家族的成员,是一种跨膜硫醇异构酶。TMX1 蛋白 可参与各种氧化还原反应,通过其活性中心二硫醇可逆氧化为二硫化物,并催化二硫醇-二硫化物交换反应。并且在细胞外 TMX1 蛋白 负调控 αibβ3 整合素的激活和血小板聚集。TMX1 蛋白, Human (HEK293, Fc) 是重组的 TMX1 蛋白,由 HEK293 表达,带有 C-mFc 标签。TMX1 蛋白, Human (HEK293, Fc) 全长 154 个氨基酸,分子量约为 ~44.4 kDa。

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描述

TMX1 Protein is a member of the protein disulfide isomerase (PDI) family and is a transmembrane thiol isomerase. TMX1 Protein can participate in various redox reactions, reversibly oxidizes to disulfide through its active center thiol, and catalyzes the thiol-disulfide exchange reaction. In addition, TMX1 Protein negatively regulates the activation of αibβ3 integrin and platelet aggregation outside the cell. TMX1 Protein, Human (HEK293, Fc) is the recombinant human-derived TMX1 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of TMX1 Protein, Human (HEK293, Fc) is 154 a.a., with molecular weight of ~44.4 kDa.

研究背景

TMX1 Protein 可参与各种氧化还原反应,既可以氧化二硫键,也可以还原二硫键,并选择性地作用于膜相关多肽[1]
TMX1 Protein 作为巯基肿瘤抑制因子,可以增加线粒体 ATP 的产生和凋亡的进展。TMX1 Protein 在氧化条件下以硫醇依赖的方式与 SERCA2b 相互作用,从而降低 SERCA 活性,从而降低内质网 Ca2+ 负荷[2]
细胞外的 TMX1 Protein 对 αibβ3 整合素的激活和血小板聚集有负调控作用[3]

种属

Human

表达系统

HEK293

标签

C-mFc

蛋白编号

AAH36460 (R27-S180)

基因 ID
蛋白结构
N-term
TMX1 (R27-S180)
Accession # AAH36460
mFc
C-term
同用名
Thioredoxin-related transmembrane protein 1; TMX; TXNDC; TXNDC1
分子量

Approximately 44.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TMX1 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TMX1 Protein, Human (HEK293, Fc)
目录号:
HY-P76681
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