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Tryptase beta-2 蛋白, Mouse (HEK293, His)

目录号: HY-P71131
产品使用指南

类胰蛋白酶 beta-2 是一种肥大细胞中丰富的中性蛋白酶,在先天免疫中发挥着关键作用。作为肥大细胞激活过程中释放的主要蛋白水解酶,类胰蛋白酶 beta-2 对免疫功能有显着贡献。它的参与强调了它在介导与先天免疫相关的细胞反应中的重要性,强调了它在免疫系统复杂的防御机制中的作用。Tryptase beta-2 蛋白, Mouse (HEK293, His) 是重组的 类胰蛋白酶 beta-2 蛋白,由 HEK293 表达,带有 C-6*His 标签。Tryptase beta-2 蛋白, Mouse (HEK293, His) 全长 255 个氨基酸,分子量为 32-38 kDa。

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规格 价格 是否有货 数量
10 μg ¥1200 询价
50 μg ¥3400 In-stock
100 μg   询价  

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MCE 顾客使用本产品发表的科研文献

  • 生物活性

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描述

Tryptase beta-2, a neutral protease abundant in mast cells, plays a key role in innate immunity. As the primary proteolytic enzyme released during mast cell activation, Tryptase beta-2 contributes significantly to immune functions. Its involvement underscores its importance in mediating cellular responses linked to innate immunity, highlighting its role in the intricate defense mechanisms of the immune system. Tryptase beta-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tryptase beta-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Tryptase beta-2 Protein, Mouse (HEK293, His) is 255 a.a., with molecular weight of 32-38 kDa.

研究背景

类胰蛋白酶 beta-2 是一种主要存在于肥大细胞中的中性蛋白酶,在先天免疫中发挥着重要作用。它是肥大细胞激活-脱粒反应过程中释放的主要蛋白水解酶,有助于其免疫功能。类胰蛋白酶 beta-2 的参与强调了它在介导与先天免疫相关的细胞反应中的重要性,强调了它在免疫系统复杂和动态防御机制中的作用。

生物活性

Data is not available.

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

P21845 (A22-S276)

基因 ID

17229  [NCBI]

蛋白结构
N-term
TPSB2 (A22-S276)
Accession # P21845
6*His
C-term
同用名
Tryptase beta-2; Tryptase-2; Mast cell protease 6; mMCP-6
氨基酸序列

APRPANQRVGIVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWVLTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHPISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGMLCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS

分子量

32-38 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM PB, 50% glycerin, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Tryptase beta-2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Tryptase beta-2 Protein, Mouse (HEK293, His)
目录号:
HY-P71131
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