UGCG 蛋白, Human (Cell-Free, His)

UGCG proteins play a key role in the glucosphingolipid (GSL) synthesis pathway based on glucosylceramide in the Golgi apparatus, catalyzing the key step of transferring glucose to ceramide to form glucosylceramide (GlcCer). GSLs have effects on membrane microdomains, affecting processes such as membrane trafficking and signal transduction. UGCG Protein, Human (Cell-Free, His) is the recombinant human-derived UGCG protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of UGCG Protein, Human (Cell-Free, His) is 394 a.a., with molecular weight of 47.7 kDa.

UGCG 蛋白在高尔基体胞质表面基于葡萄糖神经酰胺的鞘糖脂 (GSL) 合成途径中发挥着关键作用。该酶催化重要的初始步骤，将葡萄糖从 UDP-葡萄糖转移到神经酰胺，产生葡萄糖神经酰胺 (GlcCer)，这是 GSL 的基本成分。GSL 是存在于膜外层的两亲性分子，在膜微域中发挥着不可或缺的作用，影响膜运输和信号转导等过程。UGCG 的贡献延伸到关键的细胞功能，例如生长、分化、迁移和形态发生，对神经系统发育具有特定的影响。值得注意的是，GSL（包括 GlcCer）在瘦素介导的信号通路中调节瘦素受体/LEPR，对于建立皮肤屏障、调节角质形成细胞分化和组装角质化包膜是不可或缺的。此外，UGCG 促进木糖神经酰胺 (XylCer) 的合成，通过利用 UDP-Xyl 作为木糖供体，展示了其在鞘糖脂生物合成中的多功能性。这种多方面的酶活性强调了 UGCG 在多种细胞过程中的核心作用，并强调了其在维持细胞稳态和功能方面的重要性。

Human

E. coli Cell-free

N-10*His

Q16739 (M1-V394)

7357

MALLDLALEGMAVFGFVLFLVLWLMHFMAIIYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQLRGVQGGTLCFSKLDYAVAWFIRESMTIYIFLSALWDPTISWRTGRYRLRCGGTAEEILDV

47.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.