UGT1A1 蛋白, Human (P.pastoris, His)

The UGT1A1 protein is an important UDP-glucuronosyltransferase (UGT) that drives phase II biotransformation by conjugating lipophilic substrates with glucuronic acid, enhancing water solubility, and promoting excretion. UGT1A1 is critical for the elimination of drugs, xenobiotics, and endogenous compounds and exhibits multiple substrate specificities, glucuronidating estrogens, bilirubin, phytoestrogens such as genistein and daidzein, and SN -38. UGT1A1 Protein, Human (P.pastoris, His) is the recombinant human-derived UGT1A1 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of UGT1A1 Protein, Human (P.pastoris, His) is 508 a.a., with molecular weight of ~70.0 kDa.

UGT1A1 是 UDP-葡萄糖醛酸基转移酶 (UGT) 家族的重要成员，通过催化亲脂性底物与葡萄糖醛酸的结合来协调 II 期生物转化反应。这种酶促过程增强了水溶性，促进代谢物排泄到尿液或胆汁中。UGT1A1 对于药物、异生物质和内源性化合物的消除和解毒至关重要，其底物特异性具有多种用途。它催化内源性雌激素（如雌二醇、雌酮和雌三醇）的葡萄糖醛酸化，有助于调节荷尔蒙平衡。此外，UGT1A1 参与血红素降解副产物胆红素的葡萄糖醛酸化。它将其催化范围扩展到植物雌激素，例如金雀花素和大豆黄酮，以及伊立替康的药理活性代谢物 SN-38。有趣的是，虽然 UGT1A1 缺乏葡萄糖醛酸化活性，但它充当亚型 1 的负调节因子，展示了其在细胞过程中的复杂作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

P22309 (H26-H533)

54658  [NCBI]

HAGKILLIPVDGSHWLSMLGAIQQLQQRGHEIVVLAPDASLYIRDGAFYTLKTYPVPFQREDVKESFVSLGHNVFENDSFLQRVIKTYKKIKKDSAMLLSGCSHLLHNKELMASLAESSFDVMLTDPFLPCSPIVAQYLSLPTVFFLHALPCSLEFEATQCPNPFSYVPRPLSSHSDHMTFLQRVKNMLIAFSQNFLCDVVYSPYATLASEFLQREVTVQDLLSSASVWLFRSDFVKDYPRPIMPNMVFVGGINCLHQNPLSQEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH

Approximately 70.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.2 μm filtered solution in 20 mM Tris-HC1, 0.5 M NaCl, 3% Trehalose, pH 8.0.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.