1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UGT2B17 蛋白, Human (Cell-Free, His)

UGT2B17 蛋白, Human (Cell-Free, His)

目录号: HY-P702488
产品使用指南

UGT2B17 蛋白是一种关键的 UDP-葡萄糖醛酸基转移酶 (UGT),通过将亲脂性底物与葡萄糖醛酸结合来指导 II 期生物转化。该过程增强了水溶性并促进代谢物排泄到尿液或胆汁中。UGT2B17 蛋白, Human (Cell-Free, His) 是重组的 UGT2B17 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。UGT2B17 蛋白, Human (Cell-Free, His) 全长 507 个氨基酸,分子量为 64.5 kDa。

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描述

The UGT2B17 protein is a key UDP-glucuronosyltransferase (UGT) that directs phase II biotransformation by conjugating lipophilic substrates with glucuronic acid. This process enhances water solubility and promotes excretion of metabolites into urine or bile. UGT2B17 Protein, Human (Cell-Free, His) is the recombinant human-derived UGT2B17 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of UGT2B17 Protein, Human (Cell-Free, His) is 507 a.a., with molecular weight of 64.5 kDa.

研究背景

UGT2B17 是 II 期生物转化的关键参与者,协调亲脂性底物与葡萄糖醛酸的结合,这是增强水溶性并促进代谢物排泄到尿液或胆汁中的关键过程。这种多功能的 UDP-葡萄糖醛酸基转移酶 (UGT) 对内源性类固醇激素表现出特定的亲和力,包括表睾酮和雄甾酮等雄激素,以及雌二醇和表雌二醇等雌激素。通过其催化能力,UGT2B17 积极促进这些类固醇激素的葡萄糖醛酸化,塑造其生物利用度并帮助这些化合物从体内解毒和消除。UGT2B17 的酶活性强调了其在维持类固醇稳态和代谢过程的微妙平衡中的重要作用。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

O75795 (G24-D530)

基因 ID

7367

蛋白结构
N-term
10*His
UGT2B17 (G24-D530)
Accession # O75795
C-term
同用名
UDP-glucuronosyltransferase 2B17; C19-steroid-specific UDP-glucuronosyltransferase; C19-steroid-specific UDPGT
氨基酸序列

GKVLVWPTEYSHWINMKTILEELVQRGHEVIVLTSSASILVNASKSSAIKLEVYPTSLTKNDLEDFFMKMFDRWTYSISKNTFWSYFSQLQELCWEYSDYNIKLCEDAVLNKKLMRKLQESKFDVLLADAVNPCGELLAELLNIPFLYSLRFSVGYTVEKNGGGFLFPPSYVPVVMSELSDQMIFMERIKNMIYMLYFDFWFQAYDLKKWDQFYSEVLGRPTTLFETMGKAEMWLIRTYWDFEFPRPFLPNVDFVGGLHCKPAKPLPKEMEEFVQSSGENGIVVFSLGSMISNMSEESANMIASALAQIPQKVLWRFDGKKPNTLGSNTRLYKWLPQNDLLGHPKTKAFITHGGTNGIYEAIYHGIPMVGIPLFADQHDNIAHMKAKGAALSVDIRTMSSRDLLNALKSVINDPIYKENIMKLSRIHHDQPVKPLDRAVFWIEFVMRHKGAKHLRVAAHNLTWIQYHSLDVIAFLLACVATMIFMITKCCLFCFRKLAKTGKKKKRD

分子量

64.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UGT2B17 Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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