1. 重组蛋白
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  3. YidC 蛋白, E.coli (Cell-Free, His)

YidC 蛋白, E.coli (Cell-Free, His)

目录号: HY-P702491
产品使用指南

YidC 蛋白对于完整膜蛋白复合物的插入、折叠和形成至关重要。它参与膜蛋白的整合,既依赖又独立于 Sec 转位酶复合物,包括一些脂蛋白。YidC 蛋白, E.coli (Cell-Free, His) 是重组的 YidC 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。YidC 蛋白, E.coli (Cell-Free, His) 全长 548 个氨基酸,分子量为 63.0 kDa。

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描述

The YidC protein is essential for insertion, folding, and formation of integral membrane protein complexes. It is involved in the integration of membrane proteins, both dependent and independent of the Sec translocase complex, including some lipoproteins. YidC Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived YidC protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of YidC Protein, E.coli (Cell-Free, His) is 548 a.a., with molecular weight of 63.0 kDa.

研究背景

YidC 蛋白在控制细胞膜内整合膜蛋白的插入、折叠和复杂形成的复杂过程中发挥着关键作用。其多功能作用包括促进依赖 Sec 易位酶依赖性和独立途径的膜蛋白的整合,将其功能影响扩展到多种细胞过程。除了简单的插入之外,YidC 还有助于帮助多跨膜蛋白的正确折叠,从而形成对其功能至关重要的复杂三维结构。值得注意的是,YidC 与 Sec 易位酶复合体建立交互,与 SecD 等组件形成协作网络。它在整合过程中与跨膜片段的特定相互作用强调了它在膜蛋白生物发生的精心编排中的微妙参与。

种属

E.coli

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

A8A6G7 (M1-S548)

基因 ID

75205420

蛋白结构
N-term
10*His
YidC (M1-S548)
Accession # A8A6G7
C-term
同用名
Membrane protein insertase YidC; Foldase YidC; Membrane integrase YidC; Membrane protein YidC
氨基酸序列

MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAAGSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLETSPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNTFTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTFRGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYTANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLWFISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAPFALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLWFPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS

分子量

63.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

YidC Protein, E.coli (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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YidC Protein, E.coli (Cell-Free, His)
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HY-P702491
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