1. Metabolic Enzyme/Protease
  2. Aldehyde Dehydrogenase (ALDH)
  3. Win 18446

Win 18446 

目录号: HY-W011094
产品使用指南

Win 18446 是具有口服活性的睾丸特异性的酶 ALDH1a2 的抑制剂,其 IC50 值为 0.3 μM。Win 18446 可逆的抑制多个物种的精子形成,抑制睾丸内视黄醇 (HY-B1342) 合成视黄酸 (HY-14649)。

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Win 18446 Chemical Structure

Win 18446 Chemical Structure

CAS No. : 1477-57-2

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Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥1100 In-stock
50 mg ¥1000 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Description

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes[1][2].

In Vitro

WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells[2].

RT-PCR[2]

Cell Line: Testes were collected from 2-dpp mice.
Concentration: 1 μM.
Incubation Time: 24 h.
Result: Results in a VAD-like phenotype in the testis.
Alone or in combination with ROL significantly reduced Stra8 expression.
In Vivo

Win 18446 (200 mg/kg, Orally for 4, 8, and 16 weeks) significantly reduces intratesticular concentrations of retinoic acid, severely impaired spermatogenesis, and caused infertility[1].

Animal Model: Six-month-old male New Zealand white rabbits[1].
Dosage: 200 mg/kg.
Administration: Orally for 4, 8, and 16 weeks.
Result: Resulted in marked suppression of testicular weight over time.
Dramatically suppressed spermatogenesis.
Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.
Molecular Weight

366.11

Formula

C₁₂H₂₀Cl₄N₂O₂

CAS No.

1477-57-2

SMILES

O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 10.87 mg/mL (29.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7314 mL 13.6571 mL 27.3142 mL
5 mM 0.5463 mL 2.7314 mL 5.4628 mL
10 mM 0.2731 mL 1.3657 mL 2.7314 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。 -80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.09 mg/mL (2.98 mM); Clear solution

    此方案可获得 ≥ 1.09 mg/mL (2.98 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.9 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液
*以上所有助溶剂都可在 MCE 网站选购。
References
  • 摩尔计算器

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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× = ×
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Keywords:

Win 18446Win18446Win-18446Aldehyde Dehydrogenase (ALDH)Stra8TestestestisspermatogenesisInhibitorinhibitorinhibit

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