2. Apoptosis NF-κB Immunology/Inflammation
  3. TNF Receptor NF-κB Interleukin Related
  4. Anti-Mouse GITR Antibody (DTA-1)

Anti-Mouse GITR Antibody (DTA-1) 

目录号: HY-P990124 纯度: 98.47%
COA 技术支持

Anti-Mouse GITR Antibody (DTA-1) 是一种来源于大鼠靶向小鼠 GITR 的 IgG2b 单克隆激动剂抗体。Anti-Mouse GITR Antibody (DTA-1) 可以激活 T 细胞和 B 细胞以增强免疫反应。Anti-Mouse GITR Antibody (DTA-1) 通过 GITR 信号传导选择性增强 Th2 细胞效应器功能。Anti-Mouse GITR Antibody (DTA-1) 可能会加剧过敏反应。Anti-Mouse GITR Antibody (DTA-1) 可用于研究癌症和过敏性疾病，如结肠癌和过敏性哮喘。Anti-Mouse GITR Antibody (DTA-1) 对应的同型对照为大鼠 Rat IgG2b kappa, Isotype Control (HY-P990682)。

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Anti-Mouse GITR Antibody (DTA-1)

Anti-Mouse GITR Antibody (DTA-1) Chemical Structure

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Anti-Mouse GITR Antibody (DTA-1) 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Anti-Mouse GITR Antibody (DTA-1) is a rat derived IgG2b monoclonal agonist antibody targeting mouse GITR. Anti-Mouse GITR Antibody (DTA-1) can activate T cells and B cells to enhance immune response. Anti-Mouse GITR Antibody (DTA-1) selectively enhances Th2 cell effector function through GITR signaling. Anti-Mouse GITR Antibody (DTA-1) may exacerbate allergic reactions. Anti-Mouse GITR Antibody (DTA-1) can be used for research on cancer and allergic conditions such as colon cancer and allergic asthma. The recommed isotype control of Anti-Mouse GITR Antibody (DTA-1) is Rat IgG2b kappa, Isotype Control (HY-P990682)[1][2][3].

同型

Rat IgG2b
反应种属

Mouse
IC50 & Target[1][3]

NF-κB

 

IL-4

 

体外研究
(In Vitro)

Anti-Mouse GITR Antibody (DTA-1) (0.0001-100 μg/mL) 可以与 muGITR-HEK293 转染细胞系结合，EC50 为 0.14 μg/mL[1]。
Anti-Mouse GITR Antibody (DTA-1) (0.0001-100 μg/mL, 72-120 小时) 可以增强 TCR 触发的 T 细胞增殖和存活，EC50 为 0.02 μg/mL[1]。
Anti-Mouse GITR Antibody (DTA-1) 可以激活 NF-κB 信号传导，EC50 为 0.08 μg/mL[1]。
Anti-Mouse GITR Antibody (DTA-1) (5 天) 显著增加 B 细胞表面 CD86 的表达，并诱导 CD138+ 浆细胞的分化[2]。
Anti-Mouse GITR Antibody (DTA-1) (10 μg/mL, 4 天) 显著增加 Th2 细胞中 IL-4 的分泌，但对 Th1 细胞中 IFN-γ 的分泌没有影响[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse GITR Antibody (DTA-1) 相关抗体:

ELISA Assay[3]

Cell Line: CD4+CD25- T cells
Concentration: 10 μg/mL
Incubation Time: 4 days
Result: Significant increased the level of IL-4.
体内研究
(In Vivo)

Anti-Mouse GITR Antibody (DTA-1) (3.0 mg/kg, 腹腔注射, 第 7, 9 和 11 天) 显著抑制携带 MC-38 肿瘤的野生型 C57BL/6 小鼠的肿瘤生长[1]。
Anti-Mouse GITR Antibody (DTA-1) (3.0 mg/kg, 腹腔注射, 在第 0, 4 和 11 天或第 0, 4, 11 和 18 天) 在野生型 C57BL/6 小鼠 (健康或患有 MC-38 肿瘤) 中引起严重的过敏反应[1]。
Anti-Mouse GITR Antibody (DTA-1) (500 μg, 腹腔注射, 第 7 天和第 14 天) 显著抑制携带 CT26 或 EMT6 肿瘤的雌性 Balb/c 和 CD40-/- 小鼠的肿瘤生长[2]。
Anti-Mouse GITR Antibody (DTA-1) (1 mg, 腹腔注射, 单次剂量, 在第一次 OVA 激发前 1 小时) 通过选择性增强 OVA (HY-W250978) 诱导的过敏性哮喘雄性 BALB/c 小鼠的 Th2 效应器功能，加剧哮喘的气道高反应性[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 1×106 MC-38 cells injected wild type C57BL/6 mice (5-6 weeks)[1]
Dosage: 3.0 mg/kg
Administration: Intraperitoneal injection (i.p.), on day 7, 9, and 11
Result: Significantly inhibited the increase in tumor volume.
Increased the proportion of CD8+ T cells and regulatory T cells (Treg) within the tumor.
Animal Model: Wild type C57BL/6 mice (Healthy or bearing MC-38 tumors) (5-6 weeks)[1]
Dosage: 3.0 mg/kg for healthy mice, 0.1, 0.3, 1.0, 3.0, and 10.0 mg/kg for mice bearing MC-38 mice
Administration: Intraperitoneal injection (i.p.), on day 0, 4, and 11 or on day 0, 4, 11 and 18
Result: Caused a decrease in body temperature and behavioral abnormalities in mice.
Increased IL-4 and IgE levels.
Animal Model: 3×105 CT26 or 1×105 EMT6 cells injected female Balb/c and 3×105 CT26 cells injected CD40−/− mice[2]
Dosage: 500 μg
Administration: Intraperitoneal injection (i.p.), on day 7 and 14
Result: Significantly regressed 85% of the tumor in Balb/c mice and 50% of the tumor in CD40−/− mice. Increased the number of CD4+ and CD8+ T cells.
Increased the level of IFN-γ, IL-2, IL-5, and IL-10.
Increased serum IgG1 and IgG2a levels by 20 fold and 7.5 fold and generated tumor reactive IgG1 antibodies.
Animal Model: OVA (10 μg, i.p., on day 0 and 7; 10 mg/ml, inhalation, on day 21, 24 and 27) induced male BALB/c mice[3]
Dosage: 1 mg
Administration: Intraperitoneal injection (i.p.), single dose, 1 h before the first OVA challenge
Result: Significantly enhanced sensitivity to acetylcholine.
Significantly increased OVA specific IgE levels.
Had no significant change in the number of eosinophils and lymphocyte infiltration.
Significantly increased the level of IL-5, IL-10 and IL-13.
基因 ID

21936  [NCBI]

Accession

O35714-1
应用

ELISA, FACS, Functional assay, Research in vivo
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
分子量

150 kDa

性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Anti-Mouse GITR Antibody (DTA-1) 相关分类

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse GITR Antibody (DTA-1)TNF ReceptorNF-κBInterleukin RelatedTumor Necrosis Factor ReceptorTNFRNuclear factor-κBNuclear factor-kappaBILGITRT cellsB cellscolon cancerallergic asthmaMC-38CT26Inhibitorinhibitorinhibit

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