1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. ABCB5 抗体 (YA836)

ABCB5 抗体 (YA836)

目录号: HY-P80527
COA 抗体使用指南 技术支持

ABCB5 Antibody (YA836) 是一个小鼠来源、无偶联标记、抗 ABCB5 的 IgG1 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μL ¥410 In-stock
50 μL ¥1060 In-stock
100 μL ¥1700 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

ABCB5 Antibody (YA836) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCB5.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 139 kDa;
Observed band size: 90 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human ABCB5.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG1  
性状

液体

组分

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB ICC
  • Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using ABCB5 Antibody (HY-P80527) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A549 cells labeling ABCB5 with ABCB5 Antibody (HY-P80527) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ABCB5 Antibody (HY-P80527) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A549 cells labeling ABCB5 with ABCB5 Antibody (HY-P80527) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ABCB5 Antibody (HY-P80527) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:能量依赖性外排转运蛋白负责降低多药耐药细胞中的药物蓄积 (PubMed:12960149, PubMed:15205344, PubMed:15899824, PubMed:22306008)。该蛋白特异性存在于角膜缘干细胞中,并在角膜发育和修复中发挥关键作用 (基于相似性)。
亚细胞定位:细胞膜;多通道膜蛋白
表达水平:
组织特异性:在表皮黑色素细胞中,CD133 表达的祖细胞表达该蛋白 (蛋白水平) 。该蛋白广泛表达,并在色素细胞中特异性表达。在多种恶性组织中高表达:在临床黑色素瘤中高表达,在正常皮肤中低表达。在黑色素瘤中,该蛋白标记恶性黑色素瘤起始细胞 (MMIC) ,这些细胞具有无限的自我更新能力,导致肿瘤不可逆转地进展和转移,从而引发临床恶性肿瘤。该蛋白也在多种白血病细胞中高表达。在基底角膜缘上皮中也有表达。
异构体 & 翻译后修饰:Q2M3G0 有 4 个异构体:Q2M3G0-4:138641 Da (预测);Q2M3G0-2:14691 Da (预测);Q2M3G0-3:14081 Da (预测);Q2M3G0-1:89832 Da (预测)。
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
ABCB5 抗体 (YA836)
同用名
ABCB5 P-gp; ATP binding cassette sub family B (MDR/TAP) member 5; P glycoprotein ABCB5
文件资料

ABCB5 Antibody (YA836) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
ABCB5 Antibody (YA836)
目录号:
HY-P80527
需求量: