COX IV 抗体 (YA494)

COX IV Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to COX IV. It can be used as a loading control antibody.

COX4I1 是细胞色素 c 氧化酶的重要组成部分，是线粒体电子传递链中的关键成分，最终导致氧化磷酸化。该呼吸链包含三个多亚基复合物——琥珀酸脱氢酶（复合物 II、CII）、泛醇-细胞色素 c 氧化还原酶（细胞色素 b-c1 复合物、复合物 III、CIII）和细胞色素 c 氧化酶（复合物 IV、CIV）——它们协同促进电子从 NADH 和琥珀酸转移到分子氧。这个复杂的过程会在内膜上产生电化学梯度，推动跨膜运输并为 ATP 合酶提供燃料。具体来说，细胞色素 C 氧化酶协调将氧气还原为水。膜间空间中还原细胞色素 c 的电子转移涉及中间体，例如亚基 2 中的双核铜 A 中心 (CU(A)) 和亚基 1 中的血红素 A，最终汇聚在亚基 1 的活性位点（双核中心） (BNC) 由血红素 A3 和铜 B (CU(B)) 组成。BNC 利用膜间隙中细胞色素 c 的四个电子和线粒体基质中的四个质子，有效地将分子氧还原为两个水分子。因此，COX4I1 在能量代谢中发挥着核心作用，有助于复杂的氧化磷酸化过程。

Free

P13073

Predicted band size: 20 kDa；Observed band size: 15 kDa

Protein A affinity purified.

Mitochondrion inner membrane.

Non-conjugated

Unmodified

AB_3102073

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:5000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:400; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from Hela(lane 2(20μg), HepG2 (lane 3(20μg) and HEK293 (lane 4(20μg) using COX IV (HY-P80089) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of Hela cells labeling COX IV with COX IV Antibody (HY-P80089)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX IV Antibody (HY-P80089) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling COX IV with COX IV Antibody (HY-P80089) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX IV Antibody (HY-P80089) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using COX IV Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80089, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using COX IV Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80089, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human COX IV.AA range:21-70.