Proteasome 20S LMP7 抗体 (YA121)

Proteasome 20S LMP7 Antibody (YA121) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Proteasome 20S LMP7.

LMP7A 是蛋白酶体 (proteasome) 的一个亚基，参与抗原处理以生成 I 类结合肽 (class I binding peptides)。它通过替换 PSMB5 增强了免疫蛋白酶体 (immunoproteasome) 对疏水性和碱性残基后模型肽的切割能力。LMP7A 还参与生成由两个非连续蛋白片段连接而成的剪接肽 (spliced peptides)，并在干扰素 γ 诱导的敏感性中起主要作用。在凋亡过程中，它通过降解凋亡抑制因子 MCL1 发挥关键作用，并可能参与炎症反应通路。在癌细胞中，亚型 1 (E2) 被亚型 2 (E1) 替换会导致免疫蛋白酶体缺陷。此外，LMP7A 是前脂肪细胞分化为脂肪细胞的必需因子。by similar: 蛋白酶体是一种多催化蛋白复合物，能够在中性或弱碱性 pH 条件下切割特定残基 (Arg、Phe、Tyr、Leu、Glu) 相邻的肽链，并具有 ATP 依赖性蛋白水解活性。

Free

P28062

Predicted band size: 30 kDa; Observed band size: 23 kDa

Protein A affinity purified.

Nucleus. Cytoplasm.

Non-conjugated

Unmodified

AB_3102519

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

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  Immunocytochemistry analysis of Hela cells labeling Proteasome 20S LMP7 with Proteasome 20S LMP7 Antibody (HY-P80437) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Proteasome 20S LMP7 Antibody (HY-P80437)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Jurkat cells labeling Proteasome 20S LMP7 with Proteasome 20S LMP7 Antibody (HY-P80437) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Proteasome 20S LMP7 Antibody (HY-P80437) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Proteasome 20S LMP7 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat kidney tissue using Proteasome 20S LMP7 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Proteasome 20S LMP7.AA range:160-276.