1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. Proteasome 20S LMP7 抗体 (YA684)

Proteasome 20S LMP7 抗体 (YA684)

目录号: HY-P80876
COA 抗体使用指南 技术支持

Proteasome 20S LMP7 Antibody (YA684) 是一个小鼠来源、无偶联标记、抗 Proteasome 20S LMP7 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Proteasome 20S LMP7 Antibody (YA684) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Proteasome 20S LMP7.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 30 kDa;
Observed band size: 30 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human PSMB8.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG1  
性状

液体

组分

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB ICC
  • Western blot analysis of extracts from Hela(lane 2(20ug) , C6(lane 3(20ug) and Raji(lane 4(20ug) using Proteasome beta 8 Antibody (HY-P80876) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling Proteasome beta 8 Antibody (HY-P80876) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Proteasome beta 8 Antibody (HY-P80876) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling Proteasome beta 8 Antibody (HY-P80876) at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Proteasome beta 8 Antibody (HY-P80876) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:蛋白酶体是一种多催化蛋白酶复合物,其特征在于能够在中性或弱碱性 pH 条件下切割肽链,这些肽链的离去基团附近含有精氨酸 (Arg)、苯丙氨酸 (Phe)、酪氨酸 (Tyr)、亮氨酸 (Leu) 和谷氨酸 (Glu)。蛋白酶体具有 ATP 依赖性的蛋白水解活性。该亚基参与抗原加工,生成 I 类结合肽。用 PSMB8 替换 PSMB5 可增强免疫蛋白酶体切割模型肽链中疏水性和碱性残基的能力。它参与剪接肽的生成,这些剪接肽是由两个独立的蛋白酶体切割产物连接而成,这两个产物在亲代蛋白中并不相邻 (PubMed:27049119)。它是干扰素γ诱导敏感性的主要组成部分。它通过降解凋亡抑制因子 MCL1 在细胞凋亡中发挥关键作用。它可能参与炎症反应通路。在癌细胞中,异构体 1 (E2) 被异构体 2 (E1) 取代会导致免疫蛋白酶体功能缺陷。免疫蛋白酶体是前脂肪细胞分化为脂肪细胞所必需的。
亚细胞定位:细胞质;细胞核
异构体 & 翻译后修饰:P28062 有两种异构体:P28062-1:30354 Da (预测值);P28062-2:29770 Da (预测值)。
可发生自切割。成熟亚基产生的 N 端 Thr 残基负责亲核蛋白水解活性。
亚基:IFNG/IFN-γ 和 IRF1 (蛋白水平) 上调。TNF (蛋白水平) 上调。河豚毒素 (TTX) 在神经胶质细胞中上调。克罗恩病 (CD) 中上调。选择性抑制剂 PR-957 下调。HSV-1 感染在成熟树突状细胞中下调。热休克处理上调。
RRID
反应种属数据库
研究领域

Cell Biology

中文名
Proteasome 20S LMP7 抗体 (YA684)
同用名
PSMB8; LMP7; PSMB5i; RING10; Y2; Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting
文件资料

Proteasome 20S LMP7 Antibody (YA684) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Proteasome 20S LMP7 Antibody (YA684)
目录号:
HY-P80876
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