1. Epigenetics Metabolic Enzyme/Protease Anti-infection
  2. Histone Methyltransferase Phosphodiesterase (PDE) Parasite
  3. Furamidine

Furamidine  (Synonyms: DB75; NSC 305831)

目录号: HY-110137A
产品使用指南

Furamidine (DB75) 是一种选择性的且细胞可渗透的蛋白精氨酸甲基转移酶 1 (PRMT1) 抑制剂,IC50 为 9.4 μM。Furamidine 对 PRMT1 的选择性高于 PRMT5,PRMT6PRMT4 (CARM1) (IC50 分别为 166 µM,283 µM 和 >400 µM)。Furamidine 是一种有效的,可逆的和竞争性的酪氨酰 DNA 磷酸二酯酶 1 (TDP-1) 抑制剂。Furamidine 对 TDP-1 的抑制作用对单链和双链 DNA 底物均有效,对双链 DNA 的作用更强。Furamidine 也是一种抗寄生虫剂。

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Furamidine Chemical Structure

Furamidine Chemical Structure

CAS No. : 73819-26-8

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Furamidine (DB75) is a selective protein arginine methyltransferase 1 (PRMT1) inhibitor with an IC50 of 9.4 μM. Furamidine is selective for PRMT1 over PRMT5, PRMT6, and PRMT4 (CARM1) (IC50s of 166 µM, 283 µM, and >400 µM, respectively). Furamidine is a potent, reversible and competitive tyrosyl-DNA phosphodiesterase 1 (TDP-1) inhibitor. Inhibition of TDP-1 by Furamidine is effective both with single- and double-stranded DNA substrates but is slightly stronger with the duplex DNA. Furamidine is also an antiparasite agent[1][2][3].

IC50 & Target

IC50: 9.4 μM (Protein arginine methyltransferase 1 (PRMT1)); 166 µM (PRMT5), 283 µM (PRMT6) and >400 µM (PRMT4)[1]
Parasite[1]
Tyrosyl-DNA phosphodiesterase 1 (TDP-1)[2]

体外研究
(In Vitro)

Furamidine (compound 1; 20 μM; 72 hours; leukemia cell lines) inhibits cell growth for most of the leukemia cell lines except HEL cells which have JAK2V617F mutations[1].
Furamidine (compound 1; 20 μM; 15 hours; 293T cells) treatment significantly reduces the expression level of the methylated GFP-ALY protein in 293T cells[1].
Furamidine binds duplex DNA in the DNA minor groove selectively at AT rich sites [(A/T)4]. Furamidine can also intercalate between GC base pairs of duplex DNA. Furamidine could therefore interfere with DNA processing enzymes such as TDP-1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Meg-01, K562, HL-60, NB4, MOLM13, HEL, CMK, CMY, CMS and CHRF cells
Concentration: 20 μM
Incubation Time: 72 hours
Result: Inhibited cell growth for most of the leukemia cell lines except HEL cells which have JAK2V617F mutations.

Western Blot Analysis[1]

Cell Line: 293T cells
Concentration: 20 μM
Incubation Time: 15 hours
Result: The expression level of the methylated GFP-ALY protein is significantly reduced.
体内研究
(In Vivo)

Furamidine (1 mg/kg; intraperitoneal injection; 3 times a week and repeated every 4 weeks; for 34 weeks; female NZB/NZW mice) and Irinotecan combined treatment suppresses proteinuria and prolongs survival of lupus-prone NZB/NZW mice. The combination treatment does not change the levels of anti-dsDNA antibodies[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female NZB/NZW mice (6-week-old) with Irinotecan (1 mg/kg)[3]
Dosage: 1 mg/kg
Administration: Intraperitoneal injection; 3 times a week and repeated every 4 weeks; for 34 weeks
Result: Suppressed proteinuria and prolongs survival of lupus-prone NZB/NZW mice combined with Irinotecan.
分子量

304.35

Formula

C18H16N4O

CAS 号
中文名称

呋喃脒

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HY-110137A
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