1. Academic Validation
  2. STAT3-induced long noncoding RNA LINC00668 promotes migration and invasion of non-small cell lung cancer via the miR-193a/KLF7 axis

STAT3-induced long noncoding RNA LINC00668 promotes migration and invasion of non-small cell lung cancer via the miR-193a/KLF7 axis

  • Biomed Pharmacother. 2019 Aug;116:109023. doi: 10.1016/j.biopha.2019.109023.
Yun-Xia An 1 Yi-Jun Shang 1 Zhi-Wei Xu 1 Qun-Cheng Zhang 1 Zheng Wang 1 Wei-Xia Xuan 1 Xiao-Ju Zhang 2
Affiliations

Affiliations

  • 1 Department of Respiratory and Critical Care Medicine, Henan Province People's Hospital, Zhengzhou 450003, Henan, China.
  • 2 Department of Respiratory and Critical Care Medicine, Henan Province People's Hospital, Zhengzhou 450003, Henan, China. Electronic address: okksser77415@sina.com.
Abstract

Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles in non-small cell lung Cancer (NSCLC) progression. Recently, a newly identified lncRNA, LncRNA LINC00668 (LINC00668), was reported to be involved in the regulation of progression of several tumors. However, the expression pattern and biological function of LINC00668 in NSCLC remains largely unclear. In this study, we found that LINC00668 expression was significantly up-regulated in both NSCLC tissues and cell lines. we also showed that LINC00668 upregulation was induced by transcription factor STAT3. Clinical investigation demonstrated that high expression level of LINC00668 was associated with advanced TNM stage, histological grade and lymph node metastasis. Moreover, multivariate analysis confirmed LINC00668 expression level to be an independent prognostic indicator for overall survival of NSCLC patients. Functional assays indicated that knockdown of LINC00668 suppressed NSCLC cells proliferation, migration and invasion, and promoted Apoptosis. Mechanistic studies indicated that LINC00668 is a direct target of miR-193a, leading to down-regulation in the expression of its target gene KLF7. Our findings suggested that STAT3-induced LINC00668 contributed to NSCLC progression through upregulating KLF7 expression by sponging miR-193a, and may serve as a prognostic biomarker and a potential target for NSCLC.

Keywords

KLF7; LncRNA LINC00668; Non-small cell lung cancer; Prognosis; STAT3; miR-193a.

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