1. Academic Validation
  2. HSF1 promotes endometriosis development and glycolysis by up-regulating PFKFB3 expression

HSF1 promotes endometriosis development and glycolysis by up-regulating PFKFB3 expression

  • Reprod Biol Endocrinol. 2021 Jun 9;19(1):86. doi: 10.1186/s12958-021-00770-9.
Yixin Wang 1 Jing Xiu 1 Tingting Yang 1 Chune Ren 2 Zhenhai Yu 3
Affiliations

Affiliations

  • 1 Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China.
  • 2 Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China. chuneren@163.com.
  • 3 Department of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, People's Republic of China. tomsyu@163.com.
Abstract

Background: Endometriosis is a chronic hormonal inflammatory disease characterized by the presence of endometrial tissue outside the uterus. Endometriosis often causes infertility, which brings physical and mental pain to patients and their families.

Methods: We examined the functions of heat shock factor 1 (HSF1) in endometriosis development through cell count assay, cell-scratch assay and clone formation experiments. We used quantitative Real-Time PCR (qRT-PCR) and Western blot (WB) to detect HSF1 expression. Glucose and lactate levels were determined using a glucose (GO) assay kit and a lactate assay kit. Furthermore, we used a HSF1 inhibitor-KRIBB11 to establish a mouse model of endometriosis.

Results: Our data demonstrated that HSF1 promoted endometriosis development. Interestingly, HSF1 enhanced glycolysis via up-regulating PFKFB3 expression in endometriosis cells, which was a key glycolysis Enzyme. Consistently, the HSF1 Inhibitor KRIBB11 could abrogate endometriosis progression in vivo and in vitro.

Conclusions: Findings indicate that HSF1 plays an important role in endometriosis development, which might become a new target for the treatment of endometriosis.

Electronic supplementary material: Supplementary data are available.

Keywords

Endometriosis; Glycolysis; HSF1; Inhibitor; PFKFB3.

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