1. Academic Validation
  2. Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress-mediated apoptosis of bovine trophoblast cells

Replication of standard bovine viral diarrhea strain OregonC24Va induces endoplasmic reticulum stress-mediated apoptosis of bovine trophoblast cells

  • Cell Stress Chaperones. 2022 Nov 28. doi: 10.1007/s12192-022-01300-1.
Naihan Yuan # 1 Quanjiang Song # 2 Yan Jin 1 Zhenhao Zhang 1 Zheng Wu 1 Xihui Sheng 1 Xiaolong Qi 1 Kai Xing 1 Longfei Xiao 3 Xiangguo Wang 4
Affiliations

Affiliations

  • 1 Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.
  • 2 Key Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang, A&F University, Lin'an District, 666 Wusu StreetZhejiang Province, Hangzhou, 311300, China.
  • 3 Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China. xiaolf1989@bua.edu.cn.
  • 4 Animal Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China. xiangguo731@163.com.
  • # Contributed equally.
Abstract

Bovine viral diarrhea (BVD) is a worldwide infectious disease caused by bovine viral diarrhea virus (BVDV) Infection, which invades the placenta, causes abortion, produces immune tolerance and continuously infects calves, and causes huge economic losses to the cattle industry. The endoplasmic reticulum (ER) is an important organelle in cells, which is prone to ER stress after being stimulated by pathogens, thus activating the ER stress-related Apoptosis. Studies have confirmed that BVDV can utilize the ER of its host to complete its own proliferation and stimulate ER stress to a certain extent. However, the role of ER stress in BVDV infecting bovine placental trophoblast cells (BTCs) and inducing Apoptosis is still unclear. We are using the cytopathic strain of BVDV (OregonC24Va), which can cause Apoptosis of BTCs, as a model system to determine how ER stress induced by BVDV affects placental toxicity. We show that OregonC24Va can infect BTCs and proliferate in it. With the proliferation of BVDV in BTCs, ER stress-related Apoptosis is triggered. The ER stress inhibitor 4-PBA was used to inhibit the ER stress of BTCs, which not only inhibited the proliferation of BVDV, but also reduced the Apoptosis of BTCs. The ER stress activator Tg can activate ER stress-related Apoptosis, but the proliferation of BVDV does not change in BTCs. Therefore, BVDV utilizes the UPR of activated ER stress to promote the proliferation of BVDV in the early stage of Infection, and activates the ER stress-related Apoptosis of BTCs in the later stage with the virus proliferation to promote the cell Apoptosis and further spread of the virus. Our research provides a new theoretical basis for exploring the placental Infection and vertical transmission of BVDV.

Keywords

Apoptosis; BVDV; Bovine; Endoplasmic reticulum stress; Placental trophoblast cells.

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