2. Academic Validation
  3. METTL3-Mediated STING Upregulation and Activation in Kupffer Cells Contribute to Radiation-Induced Liver Disease via Pyroptosis

METTL3-Mediated STING Upregulation and Activation in Kupffer Cells Contribute to Radiation-Induced Liver Disease via Pyroptosis

  • Int J Radiat Oncol Biol Phys. 2023 Oct 30:S0360-3016(23)08046-X. doi: 10.1016/j.ijrobp.2023.10.041.
Biao Wang 1,  Yang Zhang 2,  Hao Niu 2,  Xiaomei Zhao 2,  Genwen Cheng 2,  Qianqian Zhao 2,  Guifen Ma 2,  Shisuo Du 3,  Zhaochong Zeng 4
Affiliations

Affiliations

  • 1 Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, China; Cancer center, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address: wangbiaozsh@163.com.
  • 2 Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, China; Cancer center, Zhongshan Hospital, Fudan University, Shanghai, China.
  • 3 Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, China; Cancer center, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address: du.shisuo@zs-hospital.sh.cn.
  • 4 Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai, China; Cancer center, Zhongshan Hospital, Fudan University, Shanghai, China. Electronic address: zeng.zhaochong@zs-hospital.sh.cn.
PMID: 37914138 DOI: 10.1016/j.ijrobp.2023.10.041
Abstract

Purpose: Radiation therapy is a vital adjuvant treatment for liver Cancer, although the challenge of radiation-induced liver diseases (RILDs) limits its implementation. Kupffer cells (KCs) are a crucial cell population of the hepatic immune system and their biological function can be modulated by multiple epigenetic RNA modifications, including N6-methyladenosine (m6A) methylation. However, the mechanism for m6A methylation in KC-induced inflammatory response in RILD remains unclear. The present study investigated the function of m6A modification in KCs contributing to RILD.

Methods and materials: Methylated RNA-immunoprecipitation sequencing (MeRIP-seq) and RNA transcriptome sequencing were used to explore the m6A methylation profile of primary KCs isolated from mice after irradiation with 3 × 8 Gy. Western blotting and quantitative real-time polymerase chain reaction were used to evaluate gene expression. DNA pull-down and CHIP assays were performed to verify target gene binding and identify binding site.

Results: MeRIP-seq revealed a significantly increased m6A modification level in human KCs after irradiation, suggesting the potential role of upregulated m6A in RILD. In addition, the study results corroborated that methyltransferase-like 3 (METTL3) acts as a main modulator to promote the methylation and gene expression of TEAD1, leading to STING-NLRP3 signaling activation. Importantly, it was shown that IGF2BP2 functions as an m6A "reader" to recognize methylated TEAD1 mRNA and promote its stability. METTL3/TEAD1 knockdown abolished the activation of STING-NLRP3 signaling and protected against RILD in addition to suppressing inflammatory cytokines and hepatocyte Apoptosis. Moreover, clinically collected human normal liver tissue samples post-irradiation showed increased expression of STING and IL-1β in KCs compared to the non-irradiation group. Notably, STING pharmacological inhibition alleviated irradiation-induced liver injury in mice, indicating its potential therapeutic role in RILD.

Conclusions: The results of our study reveal that TEAD1-STING-NLRP3 signaling activation contributes to RILD via METTL3-dependent m6A modification.

Keywords

METTL3,STING; NLRP3; Radiation-Induced Liver Disease(RILD); m6A.

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