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  3. MOG(35-55) amide, mouse, rat

MOG(35-55) amide, mouse, rat 

目录号: HY-P3719 纯度: 99.50%
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MOG (35-55) amide, mouse, rat,是髓鞘少突胶质细胞糖蛋白 (MOG) 免疫原性肽 35-55 片段的末端酰胺化形式 (MOG (35-55) (HY-P1240))。MOG(35-55) amide, mouse, rat 可用于实验性自身免疫性脑脊髓炎 (EAE) 模型构建。

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MOG(35-55) amide, mouse, rat

MOG(35-55) amide, mouse, rat Chemical Structure

CAS No. : 2022956-48-3

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5 mg ¥1250
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10 mg ¥2000
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25 mg ¥3300
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50 mg ¥4930
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Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

MOG (35-55) amide, mouse, rat the terminal amidation form of the 35-55 fragment of the myelin oligodendrocyte glycoprotein (MOG) immunogenic peptide (MOG (35-55) (HY-P1240)). MOG(35-55) amide, mouse, rat can be used for experimental autoimmune encephalomyelitis (EAE) modeling[1][2][3].

体外研究
(In Vitro)

扩增的 CD4+ T 细胞主要针对髓鞘少突胶质细胞糖蛋白 (MOG) 免疫原性肽 35-55 (MOG35-55),而克隆扩增的 CD8+ T 细胞对髓鞘肽或蛋白质无反应[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

MOG (35-55) amide, mouse, rat (200 μg,皮下注射,分别于第 0 天和第 7 天进行两次免疫) 可诱导 ABH 小鼠出现经典的实验性自身免疫性脑脊髓炎 (EAE) 模型,其特征是髓鞘损伤和弛缓性麻痹[2]
MOG (35-55) amide, mouse, rat (200 μg,皮下注射,分别于第 0 天和第 7 天进行两次免疫) 是 C57BL/6 小鼠中已知的脑炎诱导表位,能够诱导慢性急性加重性脑炎 (EAE),但诱导的疾病的严重程度和潜伏期表现出显著的变异性[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Biozzi ABH (H-2dq1) mice (10 weeks old) were subcutaneously injected with an emulsion mixture of antigen and complete Freund's adjuvant (CFA)[2].
Dosage: 200 μg
Administration: S.c., two immunizations were administered on day 0 and day 7
Result: Immunized with NF-L protein (200 μg each), significant spastic symptoms were observed, which could induce a classic EAE model characterized by myelin sheath damage and flaccid paralysis.
Animal Model: Female 8- to 10-week-old C57BL/6 (H-2b)[3].
Dosage: 200 μg
Administration: S.c., two immunizations were administered on day 0 and day 7
Result: The incidence of induced EAE was variable (in one experiment, 5 out of 5 mice developed the disease, with an average maximum clinical score of 3.1 (infected mice only) and a group score of 3.1; in another experiment, 3 out of 5 mice developed the disease, with an average maximum clinical score of 2.8 (infected mice) and a group score of 1.7.)
分子量

2580.97

Formula

C118H178N36O28S

CAS 号
性状

固体

颜色

White to light yellow

Sequence

Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys-NH2

Sequence Shortening

MEVGWYRSPFSRVVHLYRNGK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验: 

H2O 中的溶解度 : ≥ 100 mg/mL (38.75 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3875 mL 1.9373 mL 3.8745 mL
5 mM 0.0775 mL 0.3875 mL 0.7749 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.50%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.3875 mL 1.9373 mL 3.8745 mL 9.6863 mL
5 mM 0.0775 mL 0.3875 mL 0.7749 mL 1.9373 mL
10 mM 0.0387 mL 0.1937 mL 0.3875 mL 0.9686 mL
15 mM 0.0258 mL 0.1292 mL 0.2583 mL 0.6458 mL
20 mM 0.0194 mL 0.0969 mL 0.1937 mL 0.4843 mL
25 mM 0.0155 mL 0.0775 mL 0.1550 mL 0.3875 mL
30 mM 0.0129 mL 0.0646 mL 0.1292 mL 0.3229 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
MOG(35-55) amide, mouse, rat
目录号:
HY-P3719
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