BMPR-II 蛋白, Human (HEK293, His-Fc)

BMP Type II Receptor (BMPR2) is a type II member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. BMPR2 is highly expressed in the heart and liver, and involves in osteogenesis and cell differentiation, essential for embryogenesis, development, and adult tissue homeostasis^[1][2]. BMPR2 is associated with tubulin stability, the inhibition of BMPR2 destabilizes the microtubules promoting cell death of cancer cells that involves the activation of the lysosomes^[3]. Human BMPR2 protein contains 1038 amino acids and a transmembrane domain (151-171 a.a.). BMPR-II Protein, Human (HEK293, His-Fc) is the extracellular part of the complete BMPR2 protein, produced by HEK293 cells (M1-I151) with C-terminal hFc- and His-tag.

BMP II 型受体 (BMPR2) 是跨膜丝氨酸/苏氨酸激酶骨形态发生蛋白 (BMP) 受体家族的成员。BMP 参与软骨内骨形成和胚胎发生，通过形成两种类型 BMP 受体的异聚复合物来转导信号：I 型受体 (50-55 kD) 和 II 型受体 (70-80 kD)。II 型受体磷酸化并激活 I 型受体，I 型受体自身磷酸化，然后结合并激活 SMAD 转录调节因子^[1][2]。BMPR2 在心脏和肝脏中高表达，参与成骨和细胞分化，这对于胚胎发生、发育和成人组织稳态至关重要。BMPR2 通路抑制肺循环内平滑肌细胞 (SMC) 增殖，主要是肺小动脉内的增殖。突变后，BMPR2 与患肺动脉高压 (PAH) 的易感性增加相关^[1]。BMPR2 对于微管蛋白的稳定性至关重要，因为最近的研究表明，BMP2 独立于 BMP 1 型受体 (BMPR1) 或 Smad-1/5 转录因子来调节癌细胞中的细胞生存信号事件。BMPR2 运输蛋白的突变会导致 BMP 信号过度活跃，从而导致 BMPR2 稳定微管引起的神经系统疾病。抑制 BMPR2 会破坏微管的稳定性，从而导致溶酶体激活，从而促进癌细胞的细胞死亡进程^[3]。特别是，BMPR2启动子DNA甲基化与瓣膜性心脏病（VHD）的严重程度有很强的相关性，这使得BMPR2可以作为VHD的良好生物标志物。同时，DNA 甲基化可能通过 BMP 信号传导失调和细胞凋亡增加导致 PAH^[4]。BMPR2蛋白序列在不同物种间具有保守性，其中人类与大鼠(96.15%)和小鼠(96.63%)的序列相似度较高。

Human

HEK293

C-hFc;C-His

Q13873 (S27-I151)

659  [NCBI]

MTSSLQRPWRVPWLPWTILLVSTAAASQNQERLCAFKDPYQQDLGIGESRISHENGTILCSKGSTCYGLWEKSKGDINLVKQGCWSHIGDPQECHYEECVVTTTPPSIQNGTYRFCCCSTDLCNVNFTENFPPPDTTPLSPPHSFNRDETI

60-65 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Xu B, et al. The role of TGF-β or BMPR2 signaling pathway-related miRNA in pulmonary arterial hypertension and systemic sclerosis. Arthritis Res Ther. 2021 Nov 25;23(1):288.  [Content Brief]

  [2]. ten Dijke P, et al. Signaling via hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Curr Opin Cell Biol. 1996 Apr;8(2):139-45.  [Content Brief]

  [3]. Mondal A, et al. Bone morphogenetic protein receptor 2 inhibition destabilizes microtubules promoting the activation of lysosomes and cell death of lung cancer cells. Cell Commun Signal. 2021 Sep 25;19(1):97.  [Content Brief]

  [4]. Li N, et al. BMPR2 promoter methylation and its expression in valvular heart disease complicated with pulmonary artery hypertension. Aging (Albany NY). 2021 Nov 18;13(22):24580-24604.  [Content Brief]