1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins Protein Tyrosine Kinases
  4. CDC-like kinase 3 (CLK3)
  5. CLK3 蛋白, Human (sf9, GST)

CLK3 蛋白, Human (sf9, GST)

目录号: HY-P75678
产品使用指南

CLK3 蛋白是一种双特异性激酶,对丝氨酸/苏氨酸和含酪氨酸底物均发挥催化活性。它在剪接体复合物内磷酸化富含丝氨酸和精氨酸 (SR) 的蛋白中发挥着至关重要的作用,有助于形成控制 RNA 剪接的复杂调控机制网络。CLK3 蛋白, Human (sf9, GST) 是重组的 CLK3 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。CLK3 蛋白, Human (sf9, GST) 全长 490 个氨基酸,分子量约为 ~70 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

The CLK3 protein is a dual-specificity kinase that exerts catalytic activity on both serine/threonine and tyrosine-containing substrates. It plays a crucial role in phosphorylating serine- and arginine-rich (SR) proteins within the spliceosome complex, contributing to the complex network of regulatory mechanisms that control RNA splicing. CLK3 Protein, Human (sf9, GST) is the recombinant human-derived CLK3 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CLK3 Protein, Human (sf9, GST) is 490 a.a., with molecular weight of ~70 kDa.

研究背景

CLK3 是一种双特异性激酶,对丝氨酸/苏氨酸和含酪氨酸底物均表现出活性,特别是针对剪接体复合物中富含丝氨酸和精氨酸 (SR) 的蛋白质。这种酶是影响 SR 蛋白对 RNA 剪接控制的调节网络的组成部分,导致这些蛋白质从斑点重新分布到弥散的核质分布。CLK3 特异性磷酸化剪接机制的关键组成部分 SRSF1 和 SRSF3。此外,它在内皮细胞中组织因子 (F3) 前体 mRNA 的选择性剪接中发挥作用,表明它参与细胞过程中基因表达和剪接事件的复杂调节。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

P49761-1/NP_003983.2 (M1-R490)

基因 ID
蛋白结构
N-term
GST
CLK3 (M1-R490)
Accession # P49761
C-term
同用名
Dual specificity protein kinase CLK3; CDC-like kinase 3; CLK3
分子量

Approximately 70 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CLK3 Protein, Human (sf9, GST)
目录号:
HY-P75678
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