Flap endonuclease 1/FEN-1 蛋白, Human

Flap endonuclease 1 (FEN-1) is a structure-specific nuclease crucial for DNA replication and repair. In replication, it cleaves 5'-overhanging flaps, guiding DNA polymerase and creating a nick for ligation. FEN-1 is integral to the long patch base excision repair pathway, preventing genomic instability. It exhibits 5'-3' exonuclease and RNase H activities, playing roles in rDNA and mitochondrial DNA replication and repair. Flap endonuclease 1/FEN-1 Protein, Human is the recombinant human-derived Flap endonuclease 1/FEN-1 protein, expressed by E. coli , with tag free. The total length of Flap endonuclease 1/FEN-1 Protein, Human is 380 a.a., with molecular weight of ~44.5 kDa.

Flap 核酸内切酶 1 (FEN-1) 是一种具有双重酶活性的结构特异性核酸酶，在 DNA 复制和修复过程中发挥作用。在 DNA 复制中，FEN-1 通过切割置换合成过程中产生的 5' 悬垂瓣结构发挥着至关重要的作用，从而使 DNA 聚合酶能够导航到下游冈崎片段的 5' 端。它从 5' 端进入皮瓣并跟踪以裂解皮瓣基部，为后续结扎创建切口。此外，FEN-1 是长补丁碱基切除修复 (LP-BER) 途径的组成部分，它在无嘌呤/无嘧啶 (AP) 位点终止瓣内裂解。FEN-1 作为基因组稳定因子，可防止襟翼平衡成可能导致重复或缺失的结构。此外，该蛋白对带切口或缺口的双链 DNA 表现出 5'-3' 核酸外切酶活性，并具有 RNase H 活性。FEN-1 的多功能功能延伸至 rDNA 和线粒体 DNA 的复制和修复过程。

Biological activity has been tested in vitro.

Human

E. coli

Tag Free

P39748 (M1-K380)

2237  [NCBI]

MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK

Approximately 44.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 6% trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.