GDF-2 蛋白, Human (P. pastoris, His)

BMP-9/GDF-2 Protein, a robust angiogenesis inhibitor, selectively signals through ACVRL1 in endothelial cells. Its signaling pathway requires the TGF-beta coreceptor endoglin/ENG for efficient activation of SMAD1. Existing as a homodimer with disulfide-linked structures, the protein forms complexes with ACVRL1, BMPR2, and ACVR2B, crucial for signal transduction. Additionally, it engages in high-affinity interactions with ENG, forming a heterotetramer or a heteromeric complex with ENG and ACVRL1. Notably, it interacts with the type I receptor ACVR1, contributing to the intricate regulatory network in the TGF-beta signaling pathway. GDF-2 Protein, Human (P. pastoris, His) is the recombinant human-derived GDF-2 protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of GDF-2 Protein, Human (P. pastoris, His) is 130 a.a., with molecular weight of 16.3 kDa.

BMP-9/GDF-2 蛋白作为血管生成的有效循环抑制剂出现，特别通过 I 型激活素受体 ACVRL1 发出信号，同时排除其他 Alk。在内皮细胞中，其信号传导途径涉及 TGF-β 辅助受体内皮因子/ENG 有效激活 SMAD1 的需要。BMP-9/GDF-2 以具有二硫键连接结构的同型二聚体形式存在，在细胞外液中检测到其既作为成熟的同型二聚体又与其前肽形成复合物。该蛋白与 AVRL1、BMPR2 和 ACVR2B 建立高亲和力相互作用，这对其信号转导级联至关重要。此外，它与 ENG 形成复合物，或者作为具有 2:2 化学计量的异四聚体，或者作为与 ENG 和 AVRL1 的异聚复合物。值得注意的是，它还与 I 型受体 ACVR1 相互作用，促进 TGF-β 信号通路内复杂的调节网络。

Human

P. pastoris

N-6*His

Q9UK05 (H300-R429)

2658  [NCBI]

HEEDTDGHVAAGSTLARRKRSAGAGSHCQKTSLRVNFEDIGWDSWIIAPKEYEAYECKGGCFFPLADDVTPTKHAIVQTLVHLKFPTKVGKACCVPTKLSPISVLYKDDMGVPTLKYHYEGMSVAECGCR

16.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.